Prolactin (h): 293T Lysate: sc-170124

Prolactin (h): 293T Lysate is a vital research reagent derived from the human embryonic kidney 293T cell line, which has been transfected to express human prolactin. Prolactin is a polypeptide hormone involved in numerous physiological processes, including lactation, reproduction, and immune regulation. The 293T cell line, known for its high transfection efficiency and protein production capabilities, serves as an excellent platform for producing prolactin. In research, Prolactin (h): 293T Lysate is used to study prolactin signaling pathways, particularly the JAK2-STAT5 pathway, which is activated upon prolactin binding to its receptor. This binding triggers receptor dimerization and subsequent activation of JAK2, leading to phosphorylation of STAT5, which then translocates to the nucleus to regulate gene expression. The lysate is employed in assays to investigate prolactin's role in cell proliferation, differentiation, and apoptosis. Additionally, it is used in immunoprecipitation and Western blot experiments to identify and quantify prolactin and its associated signaling molecules. This lysate is also essential for studying the crosstalk between prolactin and other hormonal signaling pathways, providing insights into complex cellular mechanisms. The detailed analysis of prolactin's effects using this lysate advances our understanding of its diverse biological roles.