Prohibitin Antibody (E-5): sc-377037

Prohibitin Antibody (E-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Prohibitin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Prohibitin antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Prohibitin is an evolutionarily conserved protein that plays a crucial role in regulating the cell cycle, particularly by inhibiting the G1 to S phase transition, which helps maintain proper cellular proliferation and prevent uncontrolled growth. Prohibitin primarily localizes in the inner mitochondrial membrane, where this protein contributes to mitochondrial function and acts as a tumor suppressor by interacting with retinoblastoma protein and inhibiting E2F transcription factors in response to specific signaling pathways. Prohibitin also participates in B cell maturation at the plasma membrane, demonstrating diverse roles in cellular processes. Human prohibitin genes reside on chromosome 17q21 and show expression in various tissues, with expression levels fluctuating throughout the cell cycle, suggesting complex regulatory mechanisms including both transcriptional and post-translational modifications. Mutations in Prohibitin genes have been linked to breast cancer development and progression in over 80% of analyzed cell lines, highlighting significant implications in cancer biology.

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Prohibitin Antibody (E-5) References:

1. Prohibitin, a potential tumor suppressor, interacts with RB and regulates E2F function.  |  Wang, S., et al. 1999. Oncogene. 18: 3501-10. PMID: 10376528
2. Rb and prohibitin target distinct regions of E2F1 for repression and respond to different upstream signals.  |  Wang, S., et al. 1999. Mol Cell Biol. 19: 7447-60. PMID: 10523633
3. Prohibitin expression is increased in phorbol ester-treated chronic leukemic B-lymphocytes.  |  Woodlock, TJ., et al. 2001. Blood Cells Mol Dis. 27: 27-34. PMID: 11162143
4. The human prohibitin gene located on chromosome 17q21 is mutated in sporadic breast cancer.  |  Sato, T., et al. 1992. Cancer Res. 52: 1643-6. PMID: 1540973
5. Development of prohibitin ligands against osteoporosis.  |  Tabti, R., et al. 2021. Eur J Med Chem. 210: 112961. PMID: 33129591
6. Inner mitochondrial membrane protein Prohibitin 1 mediates Nix-induced, Parkin-independent mitophagy.  |  Alula, KM., et al. 2023. Sci Rep. 13: 18. PMID: 36593241
7. Efficient PHB2 (prohibitin 2) exposure during mitophagy depends on VDAC1 (voltage dependent anion channel 1).  |  Roy, M., et al. 2024. Autophagy. 1-13. PMID: 39513197
8. Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy.  |  Wang, J., et al. 2024. J Adv Res.. PMID: 39550027
9. Cell cycle activity and expression of prohibitin mRNA.  |  Roskams, AJ., et al. 1993. J Cell Physiol. 157: 289-95. PMID: 8227162
10. Prohibitin: potential role in senescence, development, and tumor suppression.  |  McClung, JK., et al. 1995. Exp Gerontol. 30: 99-124. PMID: 8591812
11. Prohibitin and the senescent phenotype.  |  Dell'Orco, RT., et al. 1996. Exp Gerontol. 31: 245-52. PMID: 8706794
12. Prohibitin in breast cancer cell lines: loss of antiproliferative activity is linked to 3' untranslated region mutations.  |  Jupe, ER., et al. 1996. Cell Growth Differ. 7: 871-8. PMID: 8809404

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Prohibitin Antibody (E-5) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-Prohibitin Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Prohibitin Antibody (E-5) Specifications

Alternate Product Name: Prohibitin 1

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 21-59

Epitope Target: Prohibitin

Epitope Species: human

Product Reactivity/Detection: Prohibitin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for Prohibitin Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin