Progesterone Receptor Double Nickase Plasmid (m): sc-422214-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Progesterone Receptor Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Progesterone Receptor Double Nickase Plasmid (m) and Progesterone Receptor Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Pgr. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Progesterone Receptor Antibody (A-2): sc-398898
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Progesterone Receptor Double Nickase Plasmid (m) | sc-422214-NIC | 20 µg | $410.00 | |||
Progesterone Receptor Double Nickase Plasmid (m2) | sc-422214-NIC-2 | 20 µg | $410.00 |
Mouse Pgr encodes the progesterone receptor, a ligand-activated nuclear hormone receptor that functions as a transcription factor to coordinate progesterone-dependent gene expression. Upon hormone binding, PGR regulates chromatin accessibility and transcriptional programs that intersect with co-regulator networks and signaling pathways controlling cell-cycle progression, differentiation, and inflammatory responses. In reproductive tissues, PGR is central to uterine receptivity, implantation, decidualization, and mammary gland development, linking endocrine cues to tissue remodeling. Dysregulated Pgr signaling and altered receptor isoform balance are widely used experimental features in models of infertility, endometrial biology, and hormone-responsive tumorigenesis.
Progesterone Receptor Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Pgr locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Pgr. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Pgr function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Pgr-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.