PRMT8 CRISPR/Cas9 KO Plasmid (h): sc-404371
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRMT8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRMT8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRMT8 CRISPR/Cas9 KO Plasmid (h) | sc-404371 | 20 µg | $397.00 |
Overview
PRMT8 (protein arginine methyltransferase 8) is a membrane-associated type I PRMT that catalyzes asymmetric dimethylation of arginine residues on protein substrates, modulating protein–protein interactions, subcellular localization, and signaling output. It contributes to regulation of post-translational modification networks that interface with cytoskeletal dynamics, vesicle trafficking, and signal transduction pathways shaped by arginine methylation. PRMT8 activity is linked to control of neuronal and synaptic processes, and altered PRMT-family methylation programs are frequently studied in contexts of neurobiology, cell differentiation, and oncogenic signaling. Dysregulated arginine methylation and PRMT-associated substrate remodeling are also investigated for their roles in stress responses and proteostasis changes observed across multiple disease-relevant models.
PRMT8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PRMT8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRMT8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRMT8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRMT8 protein expression.
This CRISPR knockout system enables efficient generation of PRMT8-deficient cell models for investigation of PRMT8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRMT8 exon(s) critical for PRMT8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRMT8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRMT8 CRISPR/Cas9 KO Plasmid (h) and PRMT8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRMT8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRMT8 HDR Plasmid (h) and PRMT8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRMT8 homology arms to support homology-directed repair at defined PRMT8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.