PRMT7 Antibody (E-9): sc-376077

PRMT7 Antibody (E-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects PRMT7 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PRMT7 (E-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PRMT7 plays a crucial role in arginine methylation, an irreversible post-translational modification essential for various cellular functions, including signal transduction, RNA transport, and RNA splicing. This modification is catalyzed by arginine methyltransferases like PRMT7, which utilizes S-adenosylmethionine as the methyl donor. PRMT7 contains two methyltransferase domains, each featuring a putative S-adenosylmethionine-binding motif, with the N-terminal domain closely resembling the catalytic core of PRMT5 and the C-terminal domain showing similarity to PRMT1. Three splice variants of PRMT7 have been identified, indicating a potential for diverse functional roles. PRMT7 is predominantly localized in the nucleus and cytoplasm, with moderate expression levels observed in adult brain and lung tissues, highlighting its importance in cellular processes and potential implications in various biological contexts.

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PRMT7 Antibody (E-9) References:

1. Prediction of the coding sequences of unidentified human genes. XXI. The complete sequences of 60 new cDNA clones from brain which code for large proteins.  |  Nagase, T., et al. 2001. DNA Res. 8: 179-87. PMID: 11572484
2. PRMT7 is a member of the protein arginine methyltransferase family with a distinct substrate specificity.  |  Miranda, TB., et al. 2004. J Biol Chem. 279: 22902-7. PMID: 15044439
3. PRMT7, a new protein arginine methyltransferase that synthesizes symmetric dimethylarginine.  |  Lee, JH., et al. 2005. J Biol Chem. 280: 3656-64. PMID: 15494416
4. A Mendelian locus on chromosome 16 determines susceptibility to doxorubicin nephropathy in the mouse.  |  Zheng, Z., et al. 2005. Proc Natl Acad Sci U S A. 102: 2502-7. PMID: 15699352
5. Protein arginine methyltransferase 6 specifically methylates the nonhistone chromatin protein HMGA1a.  |  Miranda, TB., et al. 2005. Biochem Biophys Res Commun. 336: 831-5. PMID: 16157300
6. Protein arginine (N)-methyl transferase 7 (PRMT7) as a potential target for the sensitization of tumor cells to camptothecins.  |  Verbiest, V., et al. 2008. FEBS Lett. 582: 1483-9. PMID: 18381071
7. PRMT7 methylates and suppresses GLI2 binding to SUFU thereby promoting its activation.  |  Vuong, TA., et al. 2020. Cell Death Differ. 27: 15-28. PMID: 31000813
8. PRMT7 deficiency causes dysregulation of the HCN channels in the CA1 pyramidal cells and impairment of social behaviors.  |  Lee, SY., et al. 2020. Exp Mol Med. 52: 604-614. PMID: 32269286
9. PRMT7 can prevent neurovascular uncoupling, blood-brain barrier permeability, and mitochondrial dysfunction in repetitive and mild traumatic brain injury.  |  Acosta, CH., et al. 2023. Exp Neurol. 366: 114445. PMID: 37196697
10. PRMT7 Inhibits the Proliferation and Migration of Gastric Cancer Cells by Suppressing the PI3K/AKT Pathway via PTEN.  |  Wang, X., et al. 2023. J Cancer. 14: 2833-2844. PMID: 37781082
11. PRMT-7/PRMT7 activates HLH-30/TFEB to guard plasma membrane integrity compromised by bacterial pore-forming toxins.  |  Hsieh, HC., et al. 2024. Autophagy. 20: 1335-1358. PMID: 38261662
12. Targeting PRMT7-mediated monomethylation of MAVS enhances antiviral innate immune responses and inhibits RNA virus replication.  |  Yang, J., et al. 2024. Proc Natl Acad Sci U S A. 121: e2408117121. PMID: 39546576

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PRMT7 Antibody (E-9) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-PRMT7 Antibody (E-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PRMT7 Antibody (E-9) Specifications

Alternate Product Name: PRMT7

Product Clone Name: E-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 363-401

Epitope Target: PRMT7

Epitope Species: human

Product Reactivity/Detection: PRMT7

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for PRMT7 Antibody (E-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin