PRMT6 Antibody (D-5): sc-271744

PRMT6 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects PRMT6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-PRMT6 antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PRMT6, also known as HRMT1L6, is a nuclear protein that plays a crucial role in regulating gene expression through its function as a dimethyltransferase, specifically targeting Histone H3 at arginine 2 (R2). This methylation event inhibits the trimethylation of Histone H3 at lysine 4 (K4), a modification associated with active transcription, contributing to transcriptional repression of genes typically activated by H3 K4 trimethylation. PRMT6 also methylates HIV TAT protein, suggesting a role in cellular innate immunity by restricting HIV replication. PRMT6′s ability to modulate both histone and non-histone proteins highlights its importance in various cellular processes, making PRMT6 (D-5) monoclonal antibody an essential tool for studying epigenetic regulation and viral interactions.

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PRMT6 Antibody (D-5) References:

1. Methylation of Tat by PRMT6 regulates human immunodeficiency virus type 1 gene expression.  |  Boulanger, MC., et al. 2005. J Virol. 79: 124-31. PMID: 15596808
2. Protein arginine methyltransferase 6 specifically methylates the nonhistone chromatin protein HMGA1a.  |  Miranda, TB., et al. 2005. Biochem Biophys Res Commun. 336: 831-5. PMID: 16157300
3. The AT-hook of the chromatin architectural transcription factor high mobility group A1a is arginine-methylated by protein arginine methyltransferase 6.  |  Sgarra, R., et al. 2006. J Biol Chem. 281: 3764-72. PMID: 16293633
4. Arginine methylation regulates DNA polymerase beta.  |  El-Andaloussi, N., et al. 2006. Mol Cell. 22: 51-62. PMID: 16600869
5. Arginine methylation of the human immunodeficiency virus type 1 Tat protein by PRMT6 negatively affects Tat Interactions with both cyclin T1 and the Tat transactivation region.  |  Xie, B., et al. 2007. J Virol. 81: 4226-34. PMID: 17267505
6. Arginine methylation of the HIV-1 nucleocapsid protein results in its diminished function.  |  Invernizzi, CF., et al. 2007. AIDS. 21: 795-805. PMID: 17415034
7. Methylation of histone H3R2 by PRMT6 and H3K4 by an MLL complex are mutually exclusive.  |  Guccione, E., et al. 2007. Nature. 449: 933-7. PMID: 17898714
8. Arginine methylation of the histone H3 tail impedes effector binding.  |  Iberg, AN., et al. 2008. J Biol Chem. 283: 3006-3010. PMID: 18077460
9. PRMT6-mediated methylation of R2 in histone H3 antagonizes H3 K4 trimethylation.  |  Hyllus, D., et al. 2007. Genes Dev. 21: 3369-80. PMID: 18079182
10. PRMT6 methylation of RCC1 regulates mitosis, tumorigenicity, and radiation response of glioblastoma stem cells.  |  Huang, T., et al. 2021. Mol Cell. 81: 1276-1291.e9. PMID: 33539787
11. PRMT6 methylation of STAT3 regulates tumor metastasis in breast cancer.  |  Chen, Q., et al. 2023. Cell Death Dis. 14: 655. PMID: 37813837
12. The PRMT6/STAT1/ACSL1 axis promotes ferroptosis in diabetic nephropathy.  |  Hong, J., et al. 2024. Cell Death Differ. 31: 1561-1575. PMID: 39134684

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PRMT6 Antibody (D-5) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-PRMT6 Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PRMT6 Antibody (D-5) Specifications

Alternate Product Name: PRMT6

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 181-310

Epitope Target: PRMT6

Epitope Species: human

Product Reactivity/Detection: PRMT6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for PRMT6 Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin