Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PRMT5 Double Nickase Plasmid (h): sc-401115-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRMT5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PRMT5 Double Nickase Plasmid (h) and PRMT5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PRMT5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRMT5 Antibody (A-11): sc-376937
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRMT5 Double Nickase Plasmid (h) | sc-401115-NIC | 20 µg | $410.00 | |||
PRMT5 Double Nickase Plasmid (h2) | sc-401115-NIC-2 | 20 µg | $410.00 |
PRMT5 encodes protein arginine methyltransferase 5, a major type II methyltransferase that catalyzes symmetric dimethylation of arginine residues on histones (e.g., H4R3 and H3R8) and numerous non-histone substrates. Through these methyl marks, PRMT5 helps coordinate chromatin state, transcriptional programs, and RNA metabolism, including snRNP biogenesis and pre-mRNA splicing via methylation of Sm proteins. PRMT5 activity integrates with epigenetic regulation and cell-cycle control, influencing proliferation, differentiation, and stress-response pathways. Dysregulated PRMT5 function has been associated with altered splicing and transcriptional repression signatures observed in multiple disease-relevant contexts, making it a widely studied node in gene expression control.
PRMT5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PRMT5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PRMT5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PRMT5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PRMT5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.