PRMT3 CRISPR/Cas9 KO Plasmid (h): sc-406688
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRMT3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRMT3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRMT3 CRISPR/Cas9 KO Plasmid (h) | sc-406688 | 20 µg | $397.00 |
Overview
PRMT3 encodes a type I protein arginine methyltransferase that catalyzes asymmetric dimethylation on arginine residues of protein substrates, contributing to post-translational control of protein interactions and RNA metabolism. PRMT3 is predominantly cytoplasmic and has been linked to regulation of ribosome biogenesis and translation, including methylation of ribosomal proteins, thereby influencing proteostasis and cellular growth programs. Through crosstalk with signaling and stress-response pathways, PRMT3 activity can shape gene expression outputs indirectly via translational control and assembly of ribonucleoprotein complexes. Dysregulated arginine methylation and altered PRMT family activity are frequently associated with cancer-related phenotypes and other disorders involving aberrant RNA processing and protein synthesis.
PRMT3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PRMT3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRMT3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRMT3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRMT3 protein expression.
This CRISPR knockout system enables efficient generation of PRMT3-deficient cell models for investigation of PRMT3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRMT3 exon(s) critical for PRMT3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRMT3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRMT3 CRISPR/Cas9 KO Plasmid (h) and PRMT3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRMT3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRMT3 HDR Plasmid (h) and PRMT3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRMT3 homology arms to support homology-directed repair at defined PRMT3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.