Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PRMT2 Double Nickase Plasmid (h): sc-405107-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRMT2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PRMT2 Double Nickase Plasmid (h) and PRMT2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PRMT2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRMT2 Antibody (B-11): sc-393254
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRMT2 Double Nickase Plasmid (h) | sc-405107-NIC | 20 µg | $410.00 | |||
PRMT2 Double Nickase Plasmid (h2) | sc-405107-NIC-2 | 20 µg | $410.00 |
PRMT2 encodes a type I protein arginine methyltransferase that catalyzes asymmetric dimethylation of arginine residues on histones and other nuclear proteins, shaping chromatin structure and transcriptional output. Through modulation of RNA polymerase II–linked regulatory programs and methylation-dependent protein–protein interactions, PRMT2 contributes to control of cell-cycle progression, differentiation, and cellular stress responses. PRMT2 has been implicated in crosstalk with hormone receptor signaling and NF-κB–associated transcriptional networks, linking its activity to context-dependent inflammatory and metabolic regulation. Dysregulated PRMT2 expression or activity has been associated with oncogenic transcriptional states and other complex disease phenotypes, making it a useful node for mechanistic studies of epigenetic regulation.
PRMT2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PRMT2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PRMT2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PRMT2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PRMT2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.