Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PRMT1 Double Nickase Plasmid (h): sc-402249-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRMT1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PRMT1 Double Nickase Plasmid (h) and PRMT1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PRMT1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRMT1 Antibody (B-2): sc-166963
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRMT1 Double Nickase Plasmid (h) | sc-402249-NIC | 20 µg | $410.00 | |||
PRMT1 Double Nickase Plasmid (h2) | sc-402249-NIC-2 | 20 µg | $410.00 |
PRMT1 encodes protein arginine methyltransferase 1, the major type I PRMT responsible for asymmetric dimethylation of arginine residues on histones and numerous RNA-binding and signaling proteins. Through regulation of chromatin accessibility, transcription, RNA processing, and DNA damage responses, PRMT1 contributes to core processes including cell-cycle control, apoptosis, and differentiation. PRMT1-dependent methylation interfaces with epigenetic and stress-response pathways by modulating protein–protein interactions and subcellular localization of key regulators. Dysregulated PRMT1 activity and arginine methylation patterns are frequently investigated in contexts of oncogenic transcriptional programs, inflammation, and neurodegenerative disease mechanisms.
PRMT1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PRMT1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PRMT1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PRMT1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PRMT1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.