Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PRL-3 Double Nickase Plasmid (h): sc-402331-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRL-3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PRL-3 Double Nickase Plasmid (h) and PRL-3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PTP4A3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRL-3 Antibody (318): sc-130355
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRL-3 Double Nickase Plasmid (h) | sc-402331-NIC | 20 µg | $410.00 | |||
PRL-3 Double Nickase Plasmid (h2) | sc-402331-NIC-2 | 20 µg | $410.00 |
PTP4A3 encodes the human phosphatase PRL-3 (PTP4A3), a prenylated protein tyrosine phosphatase that regulates signaling networks controlling cell migration, adhesion, and cytoskeletal remodeling. PRL-3 influences phosphorylation-dependent pathways linked to focal adhesion turnover and small GTPase signaling, supporting changes in cell polarity and invasive behavior. Dysregulated PTP4A3 expression has been associated with altered proliferative and migratory phenotypes in multiple tumor contexts and is frequently studied as a modulator of metastatic progression. As a research target, PRL-3 provides a tractable node for dissecting phosphatase-driven rewiring of kinase signaling and microenvironmental responses.
PRL-3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PTP4A3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PTP4A3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PTP4A3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PTP4A3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.