PRIM2A CRISPR/Cas9 KO Plasmid (m): sc-422397
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRIM2A CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRIM2A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRIM2A CRISPR/Cas9 KO Plasmid (m) | sc-422397 | 20 µg | $397.00 |
Overview
Prim2 encodes PRIM2A, the large catalytic subunit of DNA primase within the Pol α–primase complex that synthesizes RNA–DNA primers required to initiate DNA replication on both leading and lagging strands. PRIM2A supports replication origin firing, Okazaki fragment priming, and overall S-phase progression, and its function is tightly integrated with replisome assembly and DNA polymerase switching. Disruption of primase activity is expected to elevate replication stress, impair fork stability, and increase reliance on DNA damage response and checkpoint pathways. As a core replication factor, PRIM2A is broadly relevant to studies of genome maintenance, cell proliferation control, and mechanisms linking replication defects to genomic instability-associated disease biology.
PRIM2A CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Prim2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Prim2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Prim2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRIM2A protein expression.
This CRISPR knockout system enables efficient generation of Prim2-deficient cell models for investigation of PRIM2A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Prim2 exon(s) critical for PRIM2A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Prim2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRIM2A CRISPR/Cas9 KO Plasmid (m) and PRIM2A CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Prim2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRIM2A HDR Plasmid (m) and PRIM2A HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Prim2 homology arms to support homology-directed repair at defined Prim2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.