PRIC285 CRISPR/Cas9 KO Plasmid (h): sc-406441
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRIC285 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRIC285 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRIC285 CRISPR/Cas9 KO Plasmid (h) | sc-406441 | 20 µg | $397.00 |
Overview
HELZ2 encodes PRIC285, a putative helicase-like factor implicated in transcriptional regulation through nuclear receptor coactivation and chromatin-associated RNA metabolism. PRIC285 has been linked to metabolic gene programs governed by PPAR and related pathways, connecting it to lipid homeostasis, mitochondrial function, and inflammatory signaling. Altered HELZ2 activity has been reported in contexts of metabolic dysregulation and tumor-associated transcriptional remodeling, making it relevant for studies of gene regulatory networks and cell-state transitions. Its nuclear functions position HELZ2 as a useful node for dissecting pathway crosstalk between transcription, RNA processing, and stress-responsive programs in human cells.
PRIC285 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HELZ2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HELZ2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HELZ2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRIC285 protein expression.
This CRISPR knockout system enables efficient generation of HELZ2-deficient cell models for investigation of PRIC285 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HELZ2 exon(s) critical for PRIC285 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HELZ2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRIC285 CRISPR/Cas9 KO Plasmid (h) and PRIC285 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HELZ2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRIC285 HDR Plasmid (h) and PRIC285 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HELZ2 homology arms to support homology-directed repair at defined HELZ2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.