PRH1 CRISPR/Cas9 KO Plasmid (m): sc-422429
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRH1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRH1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRH1 CRISPR/Cas9 KO Plasmid (m) | sc-422429 | 20 µg | $397.00 |
Overview
Prh1 encodes PRH1, a secreted proline-rich protein predominantly associated with salivary gland and oral mucosal tissues in mouse. PRH1 is thought to contribute to saliva composition and local barrier functions by participating in extracellular protein networks that influence mucosal lubrication and protection. As a secreted factor with tissue-restricted expression, Prh1 is useful for studying epithelial differentiation programs and gene regulation in exocrine glands. Altered expression of salivary proteins can be informative in models of oral inflammation, salivary gland dysfunction, and environmentally induced changes to mucosal homeostasis.
PRH1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Prh1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Prh1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Prh1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRH1 protein expression.
This CRISPR knockout system enables efficient generation of Prh1-deficient cell models for investigation of PRH1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Prh1 exon(s) critical for PRH1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Prh1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRH1 CRISPR/Cas9 KO Plasmid (m) and PRH1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Prh1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRH1 HDR Plasmid (m) and PRH1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Prh1 homology arms to support homology-directed repair at defined Prh1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.