Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Prealbumin CRISPR/Cas9 KO Plasmid (h): sc-401083
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Prealbumin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Prealbumin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Prealbumin Antibody (E-1): sc-377517
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Prealbumin CRISPR/Cas9 KO Plasmid (h) | sc-401083 | 20 µg | $397.00 |
Overview
Human TTR encodes prealbumin (transthyretin), a secreted tetrameric carrier protein that transports thyroxine (T4) and retinol via binding to retinol-binding protein, supporting endocrine hormone distribution and vitamin A homeostasis. Prealbumin is synthesized predominantly by hepatocytes and also expressed in the choroid plexus, contributing to protein and hormone trafficking across plasma and cerebrospinal fluid compartments. Its biology intersects with secretory pathway proteostasis, extracellular protein turnover, and ligand-binding dynamics that influence systemic metabolic signaling. Dysregulated TTR folding and aggregation are linked to amyloid deposition disorders, while altered expression or stability can inform studies of liver function, thyroid hormone handling, and protein misfolding mechanisms.
Prealbumin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TTR gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TTR together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TTR open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Prealbumin protein expression.
This CRISPR knockout system enables efficient generation of TTR-deficient cell models for investigation of Prealbumin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TTR exon(s) critical for Prealbumin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TTR genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Prealbumin CRISPR/Cas9 KO Plasmid (h) and Prealbumin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TTR locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Prealbumin HDR Plasmid (h) and Prealbumin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TTR homology arms to support homology-directed repair at defined TTR target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.