PRDM10 Double Nickase Plasmid (h): sc-412741-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRDM10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PRDM10 Double Nickase Plasmid (h) and PRDM10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PRDM10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRDM10 Antibody (48AT1224.90.46): sc-130240
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRDM10 Double Nickase Plasmid (h) | sc-412741-NIC | 20 µg | $410.00 | |||
PRDM10 Double Nickase Plasmid (h2) | sc-412741-NIC-2 | 20 µg | $410.00 |
PRDM10 (PR domain containing 10) is a nuclear transcriptional regulator within the PR/SET domain family that helps coordinate gene expression programs linked to chromatin organization and cell-state maintenance. Through interactions with DNA-binding partners and chromatin-associated complexes, PRDM10 is positioned to influence transcriptional networks that govern proliferation, differentiation, and lineage-specific regulatory circuits. Altered PRDM family activity has been associated with dysregulated epigenetic control in cancer and developmental contexts, making PRDM10 a useful node for studying transcriptional and chromatin-dependent mechanisms. Investigating PRDM10 function supports pathway-level analyses of gene regulation, epigenomic stability, and context-dependent transcriptional outputs in human cells.
PRDM10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PRDM10 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PRDM10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PRDM10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PRDM10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.