PRAP1 CRISPR/Cas9 KO Plasmid (m): sc-423619
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRAP1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRAP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRAP1 CRISPR/Cas9 KO Plasmid (m) | sc-423619 | 20 µg | $397.00 |
Overview
Proline-rich acidic protein 1 (PRAP1), encoded by the mouse Prap1 gene, is an epithelial-associated factor enriched in gastrointestinal tissues and linked to cellular responses to luminal stress. PRAP1 expression is regulated by inflammatory and growth factor signaling, including pathways downstream of STAT and nuclear receptor networks that shape mucosal barrier function. Reported roles include modulation of epithelial survival, differentiation, and regeneration programs during tissue injury and repair. Dysregulation of PRAP1 has been associated with altered intestinal homeostasis and inflammation-related disease contexts, making it useful for probing mechanisms that connect epithelial stress adaptation to barrier integrity.
PRAP1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Prap1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Prap1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Prap1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRAP1 protein expression.
This CRISPR knockout system enables efficient generation of Prap1-deficient cell models for investigation of PRAP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Prap1 exon(s) critical for PRAP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Prap1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRAP1 CRISPR/Cas9 KO Plasmid (m) and PRAP1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Prap1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRAP1 HDR Plasmid (m) and PRAP1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Prap1 homology arms to support homology-directed repair at defined Prap1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.