PPPDE1 CRISPR/Cas9 KO Plasmid (h): sc-412095
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PPPDE1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PPPDE1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PPPDE1 CRISPR/Cas9 KO Plasmid (h) | sc-412095 | 20 µg | $397.00 |
Overview
PPPDE1 (also referenced in some contexts as DESI2) encodes a conserved deubiquitinase/peptidase-like protein implicated in post-translational regulation of protein stability and signaling through modification of ubiquitin-like conjugates. By influencing proteostasis and turnover of key regulatory proteins, PPPDE1 can affect cellular processes such as stress responses, cell-cycle control, and pathways linked to membrane and cytosolic protein quality control. Altered PPPDE1 expression and dysregulated ubiquitin-dependent signaling have been reported in cancer- and inflammation-relevant contexts, supporting its utility as a node for mechanistic studies of oncogenic signaling and cellular homeostasis. Investigating PPPDE1 loss-of-function can help define pathway dependencies and downstream substrates in human cells.
PPPDE1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DESI2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DESI2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DESI2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PPPDE1 protein expression.
This CRISPR knockout system enables efficient generation of DESI2-deficient cell models for investigation of PPPDE1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DESI2 exon(s) critical for PPPDE1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DESI2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PPPDE1 CRISPR/Cas9 KO Plasmid (h) and PPPDE1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DESI2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PPPDE1 HDR Plasmid (h) and PPPDE1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DESI2 homology arms to support homology-directed repair at defined DESI2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.