PPM1H CRISPR/Cas9 KO Plasmid (h): sc-412800
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PPM1H CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PPM1H genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PPM1H CRISPR/Cas9 KO Plasmid (h) | sc-412800 | 20 µg | $397.00 |
Overview
PPM1H encodes protein phosphatase, Mg2+/Mn2+ dependent 1H, a PP2C-family serine/threonine phosphatase that counterbalances kinase signaling by dephosphorylating target proteins involved in cellular homeostasis. Through regulation of phosphorylation-dependent pathways, PPM1H has been linked to control of cell proliferation, stress responses, and proteostasis, including processes that influence endosomal trafficking and protein turnover. Altered PPM1H expression or activity can shift signaling network dynamics and has been explored in the context of cancer-associated phospho-signaling rewiring and other disorders where phosphatase-kinase balance is disrupted. As a result, PPM1H is a useful node for mechanistic studies of phosphorylation-driven phenotypes and pathway crosstalk.
PPM1H CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PPM1H gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PPM1H together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PPM1H open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PPM1H protein expression.
This CRISPR knockout system enables efficient generation of PPM1H-deficient cell models for investigation of PPM1H signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PPM1H exon(s) critical for PPM1H function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PPM1H genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PPM1H CRISPR/Cas9 KO Plasmid (h) and PPM1H CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PPM1H locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PPM1H HDR Plasmid (h) and PPM1H HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PPM1H homology arms to support homology-directed repair at defined PPM1H target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.