PPM1H CRISPR/Cas9 KO Plasmid (m): sc-435618
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PPM1H CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PPM1H genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PPM1H CRISPR/Cas9 KO Plasmid (m) | sc-435618 | 20 µg | $397.00 |
Overview
Ppm1h encodes PPM1H, a Mg2+/Mn2+-dependent PP2C family serine/threonine phosphatase that modulates signaling output by dephosphorylating target proteins involved in cellular stress responses and growth control. Through phosphatase-driven attenuation of kinase pathways, PPM1H contributes to regulation of phosphorylation homeostasis that influences cell-cycle progression, survival, and differentiation programs. In murine systems, PPM1H has been used to interrogate how phosphatase activity shapes pathway dynamics and tissue-specific phenotypes, providing mechanistic links between altered phosphorylation networks and complex traits. Dysregulated phosphatase signaling is broadly relevant to models of oncogenic signaling, inflammation, and neurobiology, making Ppm1h a useful node for studying pathway rewiring and genotype–phenotype relationships.
PPM1H CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ppm1h gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ppm1h together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ppm1h open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PPM1H protein expression.
This CRISPR knockout system enables efficient generation of Ppm1h-deficient cell models for investigation of PPM1H signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ppm1h exon(s) critical for PPM1H function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ppm1h genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PPM1H CRISPR/Cas9 KO Plasmid (m) and PPM1H CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ppm1h locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PPM1H HDR Plasmid (m) and PPM1H HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ppm1h homology arms to support homology-directed repair at defined Ppm1h target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.