PPARγ (m): 293T Lysate: sc-122729

PPARγ (m): 293T Lysate, derived from the HEK 293T cell line, is a vital tool for investigating the Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) protein in murine systems. The HEK 293T cells, an enhanced variant of the HEK 293 cells, express the SV40 large T antigen, facilitating high-efficiency protein production. This lysate is particularly valuable for examining the molecular mechanisms by which PPARγ regulates gene expression involved in adipogenesis, glucose metabolism, and inflammation. Researchers use PPARγ (m): 293T Lysate to explore how PPARγ functions as a nuclear receptor, binding to specific PPAR response elements (PPREs) in the DNA and regulating the transcription of target genes. PPARγ forms heterodimers with the retinoid X receptor (RXR) and, upon ligand binding, undergoes conformational changes that promote the recruitment of coactivators and the transcriptional machinery. Techniques such as chromatin immunoprecipitation (ChIP), luciferase reporter assays, and electrophoretic mobility shift assays (EMSAs) are commonly employed to study these interactions and PPARγ's role in gene regulation. This lysate is instrumental in elucidating the post-translational modifications of PPARγ, such as phosphorylation and sumoylation, which affect its activity and stability. By analyzing these modifications, scientists gain insights into the regulatory mechanisms that control PPARγ's function. Research using PPARγ (m): 293T Lysate focuses strictly on fundamental biological processes, advancing our understanding of cellular metabolism and gene regulation.