PPARγ Double Nickase Plasmid (h): sc-400030-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PPARγ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PPARγ Double Nickase Plasmid (h) and PPARγ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PPARG. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PPARγ Antibody (E-8): sc-7273
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PPARγ Double Nickase Plasmid (h) | sc-400030-NIC | 20 µg | $410.00 | |||
PPARγ Double Nickase Plasmid (h2) | sc-400030-NIC-2 | 20 µg | $410.00 |
PPARG encodes peroxisome proliferator-activated receptor gamma (PPARγ), a ligand-activated nuclear receptor that heterodimerizes with RXR to regulate transcriptional programs controlling adipogenesis, lipid uptake and storage, and insulin sensitivity. PPARγ integrates metabolic and inflammatory cues by modulating pathways such as fatty acid metabolism, glucose homeostasis, and macrophage polarization, influencing cytokine signaling and tissue remodeling. Altered PPARG activity or expression is linked to metabolic dysfunction, including obesity, insulin resistance, and dyslipidemia, and is also studied in contexts such as hepatic steatosis and inflammatory disorders. Because it functions as a transcriptional hub, PPARG is frequently interrogated to map gene regulatory networks and metabolic state transitions in human cell models.
PPARγ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PPARG locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PPARG. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PPARG function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PPARG-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.