PPA1 Antibody (B-8): sc-377081

PPA1 Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects PPA1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PPA1 (B-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PPA1, also known as pyrophosphate phospho-hydrolase 1 or inorganic pyrophosphatase, plays a crucial role in phosphate metabolism by catalyzing the conversion of pyrophosphate to inorganic phosphate, a vital reaction for cellular energy homeostasis. PPA1 is predominantly located in the cytoplasm and is essential for cell growth, as PPA1 facilitates the availability of inorganic phosphate necessary for various biochemical processes. Structurally, PPA1 exists as a homodimer, with each subunit capable of binding magnesium ions, which are critical for enzymatic activity. The binding of two magnesium ions is required to activate PPA1, while additional magnesium ions can form complexes with substrates and products, enhancing the efficiency of the reaction. Notably, PPA1 activity can be inhibited by calcium, highlighting the importance of ion balance in regulating function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PPA1 Antibody (B-8) References:

1. Cloning and expression profile of human inorganic pyrophosphatase.  |  Fairchild, TA. and Patejunas, G. 1999. Biochim Biophys Acta. 1447: 133-6. PMID: 10542310
2. Cyclopentenone isoprostanes induced by reactive oxygen species trigger defense gene activation and phytoalexin accumulation in plants.  |  Thoma, I., et al. 2003. Plant J. 34: 363-75. PMID: 12713542
3. Computer modeling of two inorganic pyrophosphatases.  |  Vihinen, M., et al. 1992. Biochem Biophys Res Commun. 186: 122-8. PMID: 1321599
4. Human mitochondrial pyrophosphatase: cDNA cloning and analysis of the gene in patients with mtDNA depletion syndromes.  |  Curbo, S., et al. 2006. Genomics. 87: 410-6. PMID: 16300924
5. Localization of the structural genes for hexokinase-1 and inorganic pyrophosphatase on region (pter-->q24) of human chromosome 10.  |  Chern, CJ. 1976. Cytogenet Cell Genet. 17: 338-42. PMID: 17494625
6. Studies on human erythrocyte inorganic pyrophosphatase.  |  Fisher, RA., et al. 1974. Ann Hum Genet. 37: 341-53. PMID: 4130389
7. Further studies on erythrocyte inorganic pyrophosphatase: an examination of different mammalian species and human-Chinese hamster hybrid cells.  |  Fisher, RA., et al. 1974. Ann Hum Genet. 38: 171-8. PMID: 4219723
8. Assignment of the inorganic pyrophosphatase gene locus (PP) to chromosome 10 in man.  |  McAlpine, PJ., et al. 1976. Cytogenet Cell Genet. 16: 201-3. PMID: 975879

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PPA1 Antibody (B-8) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PPA1 Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PPA1 Antibody (B-8) Specifications

Alternate Product Name: PPA1

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 228-289

Epitope Target: PPA1

Epitope Species: human

Product Reactivity/Detection: PPA1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PPA1 Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin