PP2A-B56-δ Antibody (H5D12): sc-81605

PP2A-B56-δ Antibody (H5D12) is a mouse monoclonal IgG1 kappa light chain antibody that detects PP2A-B56-delta protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-PP2A-B56-delta antibody (H5D12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PP2A-B56-delta protein plays a crucial role in cellular processes through involvement in the protein phosphatase 2A (PP2A) complex, which dephosphorylates serine and threonine residues on target proteins. This dephosphorylation maintains cellular homeostasis, regulates cell division, and controls apoptosis, making PP2A-B56-delta essential in various signaling pathways. PP2A-B56-delta′s structure, characterized by trimeric assembly with catalytic and regulatory subunits, enables specific interactions with diverse substrates, influencing numerous cellular functions. Understanding PP2A-B56-delta dynamics and interactions helps elucidate roles in health and disease, particularly in cancer and neurodegenerative disorders, where dysregulated phosphatase activity can lead to pathological consequences.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PP2A-B56-δ Antibody (H5D12) References:

1. Structure and expression of two isoforms of the murine calmodulin-dependent protein phosphatase regulatory subunit (calcineurin B).  |  Ueki, K., et al. 1992. Biochem Biophys Res Commun. 187: 537-43. PMID: 1325794
2. PP2A-AMPKα-HSF1 axis regulates the metal-inducible expression of HSPs and ROS clearance.  |  Zhu, XN., et al. 2014. Cell Signal. 26: 825-32. PMID: 24412756
3. Combination of Hypoglycemia and Metformin Impairs Tumor Metabolic Plasticity and Growth by Modulating the PP2A-GSK3β-MCL-1 Axis.  |  Elgendy, M., et al. 2019. Cancer Cell. 35: 798-815.e5. PMID: 31031016
4. Growth arrest of PPP2R5C and PPP2R5D double knockout mice indicates a genetic interaction and conserved function for these PP2A B subunits.  |  Dyson, JJ., et al. 2022. FASEB Bioadv. 4: 273-282. PMID: 35415460
5. Extended regulation interface coupled to the allosteric network and disease mutations in the PP2A-B56δ holoenzyme.  |  Wu, CG., et al. 2023. bioRxiv.. PMID: 37066309
6. B56δ long-disordered arms form a dynamic PP2A regulation interface coupled with global allostery and Jordan's syndrome mutations.  |  Wu, CG., et al. 2024. Proc Natl Acad Sci U S A. 121: e2310727120. PMID: 38150499
7. Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing.  |  Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293
8. Serine/threonine protein phosphatases.  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
9. Important roles for novel protein phosphatases dephosphorylating serine and threonine residues.  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
10. A regulatory subunit of smooth muscle myosin bound phosphatase.  |  Okubo, S., et al. 1994. Biochem Biophys Res Commun. 200: 429-34. PMID: 8166716
11. Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing.  |  Hendrix, P., et al. 1993. J Biol Chem. 268: 15267-76. PMID: 8392071
12. Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth.  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PP2A-B56-δ Antibody (H5D12) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-PP2A-B56-δ Antibody (H5D12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PP2A-B56-δ Antibody (H5D12) Specifications

Alternate Product Name: PP2A phosphatase regulatory subunit B56 delta

Product Clone Name: H5D12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-165

Epitope Target: PP2A-B56-δ

Epitope Species: human

Product Reactivity/Detection: PP2A-B56-δ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for PP2A-B56-δ Antibody (H5D12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin