PP1β Antibody (C-5): sc-373782
- PP1β Antibody (C-5) is a mouse monoclonal IgG1 κ, cited in 9 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 295-322 at the C-terminus of PP1β of human origin
- Anti-PP1 beta Antibody (C-5) is recommended for detection of PP1β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-PP1 beta Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PP1β Antibody (C-5) for WB and IHC(P) applications. These reagents are now offered in bundles with PP1β Antibody (C-5) (see ordering information below).
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PP1β Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody engineered to recognize an epitope spanning amino acids 295-322 at the C-terminus of human Protein Phosphatase 1 Beta (PP1β), also known as PPP1CB. PP1β monoclonal antibody (C-5) exhibits robust species reactivity, effectively detecting PP1β from mouse, rat, and human origins through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), while also showing cross-reactivity with additional species such as canine, bovine, porcine, and avian. Available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, anti-PP1β antibody (C-5) provides versatile options to accommodate diverse experimental requirements. PP1β is a pivotal catalytic subunit within the Protein Phosphatase 1 (PP1) family, characterized by its conserved catalytic domain essential for serine/threonine phosphatase activity and a unique C-terminal region that mediates interactions with regulatory and targeting proteins. Post-translational modifications (PTMs) of PP1β, such as phosphorylation, methylation, and ubiquitination, play critical roles in modulating activity, stability, and subcellular localization, thereby fine-tuning involvement in key cellular processes including cell cycle regulation, metabolism, and apoptosis. Phosphorylation of PP1β can alter enzymatic activity and influence binding affinity to regulatory subunits, thereby impacting ability to dephosphorylate specific substrates. Methylation and ubiquitination further regulate PP1β by affecting interactions with other proteins and degradation via the proteasome pathway, respectively. Understanding these PTMs is crucial as they provide mechanisms for precise control of PP1β function, ensuring accurate modulation of phosphorylation states within the cell and facilitating appropriate responses to various signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PP1β Antibody (C-5) References:
- Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing. | Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293
- Serine/threonine protein phosphatases. | Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
- Important roles for novel protein phosphatases dephosphorylating serine and threonine residues. | Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
- A regulatory subunit of smooth muscle myosin bound phosphatase. | Okubo, S., et al. 1994. Biochem Biophys Res Commun. 200: 429-34. PMID: 8166716
- Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing. | Hendrix, P., et al. 1993. J Biol Chem. 268: 15267-76. PMID: 8392071
- Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth. | Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PP1β Antibody (C-5) | sc-373782 | 200 µg/ml | $322.00 | |||
PP1β Antibody (C-5): m-IgG Fc BP-HRP Bundle | sc-527412 | 200 µg Ab; 10 µg BP | $361.00 | |||
PP1β Antibody (C-5): m-IgG1 BP-HRP Bundle | sc-532785 | 200 µg Ab; 20 µg BP | $361.00 | |||
PP1β Antibody (C-5) AC | sc-373782 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PP1β Antibody (C-5) HRP | sc-373782 HRP | 200 µg/ml | $322.00 | |||
PP1β Antibody (C-5) FITC | sc-373782 FITC | 200 µg/ml | $336.00 | |||
PP1β Antibody (C-5) PE | sc-373782 PE | 200 µg/ml | $349.00 | |||
PP1β Antibody (C-5) Alexa Fluor® 488 | sc-373782 AF488 | 200 µg/ml | $364.00 | |||
PP1β Antibody (C-5) Alexa Fluor® 546 | sc-373782 AF546 | 200 µg/ml | $364.00 | |||
PP1β Antibody (C-5) Alexa Fluor® 594 | sc-373782 AF594 | 200 µg/ml | $364.00 | |||
PP1β Antibody (C-5) Alexa Fluor® 647 | sc-373782 AF647 | 200 µg/ml | $364.00 | |||
PP1β Antibody (C-5) Alexa Fluor® 680 | sc-373782 AF680 | 200 µg/ml | $364.00 | |||
PP1β Antibody (C-5) Alexa Fluor® 790 | sc-373782 AF790 | 200 µg/ml | $364.00 | |||
PP1β (C-5) Neutralizing Peptide | sc-373782 P | 100 µg/0.5 ml | $69.00 |