Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
POPX2 CRISPR/Cas9 KO Plasmid (h): sc-406536
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- POPX2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the POPX2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: POPX2 Antibody (E-2): sc-514894
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
POPX2 CRISPR/Cas9 KO Plasmid (h) | sc-406536 | 20 µg | $397.00 |
Overview
PPM1F encodes the serine/threonine protein phosphatase POPX2 (PP2C family), a regulator of phosphorylation-dependent signaling that counterbalances kinase pathways controlling cytoskeletal dynamics and cell-cycle–associated processes. POPX2 has been linked to modulation of actin remodeling and cell motility through dephosphorylation of select substrates within Rho GTPase–coupled and stress-responsive networks, integrating cues that influence adhesion and directional migration. By tuning these signaling nodes, POPX2 can shape cellular responses such as proliferation, survival, and invasive behavior in model systems. Dysregulated phosphatase activity and altered PPM1F/POPX2 expression have been reported in contexts relevant to cancer biology and other disorders where aberrant phosphorylation signaling perturbs tissue homeostasis.
POPX2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PPM1F gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PPM1F together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PPM1F open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish POPX2 protein expression.
This CRISPR knockout system enables efficient generation of PPM1F-deficient cell models for investigation of POPX2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PPM1F exon(s) critical for POPX2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PPM1F genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by POPX2 CRISPR/Cas9 KO Plasmid (h) and POPX2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PPM1F locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by POPX2 HDR Plasmid (h) and POPX2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PPM1F homology arms to support homology-directed repair at defined PPM1F target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.