POGK Double Nickase Plasmid (h): sc-412865-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- POGK Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- POGK Double Nickase Plasmid (h) and POGK Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting POGK. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: POGK Antibody (F-1): sc-515752
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
POGK Double Nickase Plasmid (h) | sc-412865-NIC | 20 µg | $410.00 |
POGK (pogo transposable element derived with KRAB domain) encodes a nuclear protein derived from a pogo-like transposase and is thought to contribute to DNA-binding–mediated regulation of chromatin state and transcription. The presence of a KRAB domain suggests potential recruitment of KAP1/TRIM28-associated co-repressor complexes, linking POGK to heterochromatin formation and epigenetic control of gene expression. Altered regulation of KRAB-containing factors is broadly relevant to genome stability, developmental programs, and cell-state transitions, making POGK a useful target for mechanistic studies of transcriptional repression and chromatin organization. Investigating POGK perturbation can help clarify how transposase-derived proteins are repurposed within human regulatory networks and how these mechanisms intersect with disease-associated transcriptional dysregulation.
POGK Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the POGK locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within POGK. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt POGK function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of POGK-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.