PNAd Antibody (E-11): sc-166493

PNAd Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects PNAd in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PNAd antibody (E-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PNAd, also known as Protein N-terminal asparagine amidohydrolase, consists of 310 amino acids and is encoded by the human NTAN1 gene. PNAd plays a crucial role in the side-chain deamidation of N-terminal asparagine residues to aspartate, a process essential for the ubiquitin-dependent degradation of intracellular proteins that begin with a methionine-asparagine sequence. The conversion of N-terminal asparagine to aspartate by PNAd facilitates subsequent arginylation and polyubiquitination of these proteins, marking them for degradation according to the N-end rule, thereby regulating protein turnover and maintaining cellular homeostasis. PNAd does not act on substrates with internal or C-terminal asparagines, nor does PNAd modify glutamine residues, highlighting specificity and significance in cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PNAd Antibody (E-11) References:

1. Altered activity, social behavior, and spatial memory in mice lacking the NTAN1p amidase and the asparagine branch of the N-end rule pathway.  |  Kwon, YT., et al. 2000. Mol Cell Biol. 20: 4135-48. PMID: 10805755
2. Varying intertrial interval reveals temporally defined memory deficits and enhancements in NTAN1-deficient mice.  |  Balogh, SA., et al. 2000. Learn Mem. 7: 279-86. PMID: 11040259
3. Facilitated stimulus-response associative learning and long-term memory in mice lacking the NTAN1 amidase of the N-end rule pathway.  |  Balogh, SA., et al. 2001. Brain Res. 892: 336-43. PMID: 11172781
4. Behavioral characterization of mice lacking the ubiquitin ligase UBR1 of the N-end rule pathway.  |  Balogh, SA., et al. 2002. Genes Brain Behav. 1: 223-9. PMID: 12882367
5. Stimulation of ubiquitin-proteasome pathway through the expression of amidohydrolase for N-terminal asparagine (Ntan1) in cultured rat hippocampal neurons exposed to static magnetism.  |  Hirai, T., et al. 2006. J Neurochem. 96: 1519-30. PMID: 16539681
6. The magnetism responsive gene Ntan1 in mouse brain.  |  Goto, Y., et al. 2006. Neurochem Int. 49: 334-41. PMID: 16600435
7. A mouse amidase specific for N-terminal asparagine. The gene, the enzyme, and their function in the N-end rule pathway.  |  Grigoryev, S., et al. 1996. J Biol Chem. 271: 28521-32. PMID: 8910481

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PNAd Antibody (E-11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PNAd Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PNAd Antibody (E-11) Specifications

Alternate Product Name: PNAd

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 171-310

Epitope Target: PNAd

Epitope Species: human

Product Reactivity/Detection: PNAd

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PNAd Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin