Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PMP22 Double Nickase Plasmid (m): sc-422323-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PMP22 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PMP22 Double Nickase Plasmid (m) and PMP22 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Pmp22. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PMP22 Antibody (G-6): sc-515199
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PMP22 Double Nickase Plasmid (m) | sc-422323-NIC | 20 µg | $410.00 |
Peripheral myelin protein 22 (PMP22), encoded by the mouse Pmp22 gene, is a tetraspan membrane glycoprotein highly enriched in Schwann cells and compact myelin, where it helps regulate myelin membrane composition, stability, and peripheral nerve conduction. PMP22 participates in membrane trafficking and proteostasis pathways, including ER quality control and endosomal–lysosomal turnover, linking its abundance to myelin homeostasis and Schwann cell differentiation programs. Altered PMP22 dosage or misfolding is tightly associated with peripheral neuropathy phenotypes, making Pmp22 a key node for studying myelination, axon–glia interactions, and stress-response signaling in the peripheral nervous system.
PMP22 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Pmp22 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Pmp22. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Pmp22 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Pmp22-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.