PMF-1 Antibody (D-6): sc-376245

PMF-1 Antibody (D-6) is a mouse monoclonal IgG1 antibody that detects PMF-1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PMF-1, or polyamine-modulated factor 1, is a crucial 205 amino acid protein primarily localized in the nucleus, where PMF-1 plays a significant role in kinetochore formation, a process essential for accurate chromosome segregation during cell division. PMF-1 features a coiled-coil domain that facilitates interaction with the leucine-zipper domain of Nrf2, thereby regulating the transcription of SSAT, an enzyme pivotal for polyamine catabolism. This regulation is vital as PMF-1 influences cellular growth and differentiation, particularly in tissues with high metabolic demands. PMF-1 is a key component of the MIS12 complex, which is indispensable for proper chromosomal alignment and segregation, ensuring genomic stability. Notably, PMF-1 is expressed at elevated levels in skeletal muscle and heart tissues, with moderate expression observed in the liver and kidney, and PMF-1 exists in five isoforms generated through alternative splicing, highlighting functional diversity and regulatory potential in various biological contexts.

The preferred PMF-1 antibody is PMF-1 Antibody (B-5): sc-376988.