Anti-PLTP Antibody (A-12) recommended for detection of precursor and mature forms of isoforms 1 and 2 of PLTP of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PLTP Antibody (A-12): sc-271596

Anti-PLTP Antibody (A-12) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-PLTP Antibody (A-12) is a mouse monoclonal IgG2a κ PLTP antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 221-493 mapping at the C-terminus of PLTP of human origin
    • PLTP Antibody (A-12) is recommended for detection of precursor and mature forms of isoforms 1 and 2 of PLTP of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-PLTP Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PLTP (A-12): sc-271596.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PLTP Antibody (A-12) for WB and IHC(P) applications. These reagents are now offered in bundles with PLTP Antibody (A-12) (see ordering information below).
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Can PLTP (A-12): sc-271596 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, PLTP (A-12): sc-271596 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic staining Excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-03-14
Rated 5 out of 5 by from Satisfactory Western Blot data of PLTP expression Satisfactory Western Blot data of PLTP expression in non-transfected and human PLTP transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-04-14
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