PLIC-2 Double Nickase Plasmid (m): sc-424895-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PLIC-2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PLIC-2 Double Nickase Plasmid (m) and PLIC-2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ubqln2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PLIC-2 Antibody (QR-2): sc-100612
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLIC-2 Double Nickase Plasmid (m) | sc-424895-NIC | 20 µg | $410.00 |
Mouse Ubqln2 encodes PLIC-2 (ubiquilin-2), a ubiquitin-like/ubiquitin-associated adaptor that couples polyubiquitinated substrates to the 26S proteasome and supports protein quality control. PLIC-2 participates in ubiquitin–proteasome system flux, ER-associated degradation, and the turnover of misfolded or aggregated proteins, influencing cellular stress responses and proteostasis. Through interactions with proteasomal subunits and ubiquitin chains, it helps regulate degradation-linked signaling and can affect autophagy–proteasome crosstalk under proteotoxic stress. Dysregulation of UBQLN2 family function has been linked to protein aggregation phenotypes and neurodegeneration-relevant pathways, making Ubqln2 perturbation useful for mechanistic studies of neuronal proteostasis and stress signaling.
PLIC-2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ubqln2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ubqln2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ubqln2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ubqln2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.