PLEKHG5 Antibody (5A9): sc-517108
- PLEKHG5 Antibody (5A9) is a mouse monoclonal IgG2a κ PLEKHG5 antibody provided at 100 µg/ml
- raised against amino acids 896-995 representing partial length PLEKHG5 of human origin
- recommended for detection of PLEKHG5 of human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PLEKHG5 Antibody (5A9). This work is in progress.
PLEKHG5 Antibody (5A9) is a mouse monoclonal IgG2a kappa light chain antibody that detects PLEKHG5 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-PLEKHG5 antibody (5A9) is available as the non-conjugated form. PLEKHG5, also known as guanine nucleotide exchange factor 720 (GEF720) or DSMA4, plays a crucial role in cellular signaling, particularly in the activation of the NFκB pathway, which is essential for regulating immune responses and cell survival. PLEKHG5 is predominantly expressed in the peripheral nervous system and brain, where PLEKHG5 is localized in the cytoplasm; however, upon cellular stimulation, PLEKHG5 translocates to perinuclear regions, highlighting its dynamic role in cellular signaling. The presence of the highly-conserved DH-PH module in PLEKHG5 is significant as this module serves as a hallmark of the Dbl family of guanine nucleotide exchange factors, facilitating the exchange of GDP for GTP on Rho family GTPases, which are critical for various cellular processes including cytoskeletal dynamics and cell differentiation. Mutations in the PLEKHG5 gene have been implicated in autosomal recessive distal spinal muscular atrophy (DSMA), underscoring PLEKHG5′s relevance in neurodegenerative diseases. Four isoforms of PLEKHG5 exist due to alternative splicing, which may contribute to PLEKHG5′s functional diversity and regulatory mechanisms in different cellular contexts.
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PLEKHG5 Antibody (5A9) References:
- Signaling to the Rho GTPases: networking with the DH domain. | Hoffman, GR. and Cerione, RA. 2002. FEBS Lett. 513: 85-91. PMID: 11911885
- Critical role of the pleckstrin homology domain in Dbs signaling and growth regulation. | Fuentes, EJ., et al. 2003. J Biol Chem. 278: 21188-96. PMID: 12637530
- Substrate specificity and recognition is conferred by the pleckstrin homology domain of the Dbl family guanine nucleotide exchange factor P-Rex2. | Joseph, RE. and Norris, FA. 2005. J Biol Chem. 280: 27508-12. PMID: 15897194
- The Dbs PH domain contributes independently to membrane targeting and regulation of guanine nucleotide-exchange activity. | Baumeister, MA., et al. 2006. Biochem J. 400: 563-72. PMID: 17007612
- The nuclear factor kappaB-activator gene PLEKHG5 is mutated in a form of autosomal recessive lower motor neuron disease with childhood onset. | Maystadt, I., et al. 2007. Am J Hum Genet. 81: 67-76. PMID: 17564964
- Crucial structural role for the PH and C1 domains of the Vav1 exchange factor. | Rapley, J., et al. 2008. EMBO Rep. 9: 655-61. PMID: 18511940
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLEKHG5 Antibody (5A9) | sc-517108 | 100 µg/ml | $322.00 |