PLEKHA4 Antibody (H-1): sc-376408
- PLEKHA4 Antibody (H-1) is a mouse monoclonal IgG1 κ PLEKHA4 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 427-459 within an internal region of PLEKHA4 of human origin
- PLEKHA4 Antibody (H-1) is recommended for detection of PLEKHA4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-PLEKHA4 Antibody (H-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for PLEKHA4 Antibody (H-1) for WB and IHC(P) applications. This reagent is now offered in a bundle with PLEKHA4 Antibody (H-1) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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PLEKHA4 Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects PLEKHA4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PLEKHA4 (H-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PLEKHA4, also known as Pleckstrin homology domain-containing family A member 4 or PEPP1 (Phosphoinositol 3-phosphate-binding protein 1), is a 779 amino acid protein characterized by a single Pleckstrin homology (PH) domain, which plays a crucial role in intracellular signaling pathways. PLEKHA4 specifically binds to phosphatidylinositol-3-phosphate (PtdIns3P), a phospholipid predominantly located on early endosomes, facilitating endosomal trafficking and signaling regulation. PLEKHA4 shows high expression in melanoma, potentially contributing to tumor progression and metastasis, while maintaining low expression in normal tissues such as skeletal muscle, small intestine, liver, heart, and kidney, suggesting a role in cancer-specific pathways. PLEKHA4 gene is located on human chromosome 19, known for high gene density, and produces two isoforms through alternative splicing, which may have distinct functional roles in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PLEKHA4 Antibody (H-1) References:
- Disease genes and chromosomes: disease maps of the human genome. Chromosome 19. | Gilbert, F. 1997. Genet Test. 1: 145-9. PMID: 10464639
- Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities. | Dowler, S., et al. 2000. Biochem J. 351: 19-31. PMID: 11001876
- Molecular determinants in pleckstrin homology domains that allow specific recognition of phosphoinositides. | Lemmon, MA. and Ferguson, KM. 2001. Biochem Soc Trans. 29: 377-84. PMID: 11497993
- Membrane targeting by pleckstrin homology domains. | Cozier, GE., et al. 2004. Curr Top Microbiol Immunol. 282: 49-88. PMID: 14594214
- The DNA sequence and biology of human chromosome 19. | Grimwood, J., et al. 2004. Nature. 428: 529-35. PMID: 15057824
- Pleckstrin homology domains: not just for phosphoinositides. | Lemmon, MA. 2004. Biochem Soc Trans. 32: 707-11. PMID: 15493994
- Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells. | Subramanian, D., et al. 2010. Nat Chem Biol. 6: 324-6. PMID: 20364126
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLEKHA4 Antibody (H-1) | sc-376408 | 200 µg/ml | $316.00 | |||
PLEKHA4 Antibody (H-1): m-IgGκ BP-HRP Bundle | sc-522947 | 200 µg Ab, 40 µg BP | $354.00 | |||
PLEKHA4 Antibody (H-1) AC | sc-376408 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PLEKHA4 Antibody (H-1) HRP | sc-376408 HRP | 200 µg/ml | $316.00 | |||
PLEKHA4 Antibody (H-1) FITC | sc-376408 FITC | 200 µg/ml | $330.00 | |||
PLEKHA4 Antibody (H-1) PE | sc-376408 PE | 200 µg/ml | $343.00 | |||
PLEKHA4 Antibody (H-1) Alexa Fluor® 488 | sc-376408 AF488 | 200 µg/ml | $357.00 | |||
PLEKHA4 Antibody (H-1) Alexa Fluor® 546 | sc-376408 AF546 | 200 µg/ml | $357.00 | |||
PLEKHA4 Antibody (H-1) Alexa Fluor® 594 | sc-376408 AF594 | 200 µg/ml | $357.00 | |||
PLEKHA4 Antibody (H-1) Alexa Fluor® 647 | sc-376408 AF647 | 200 µg/ml | $357.00 | |||
PLEKHA4 Antibody (H-1) Alexa Fluor® 680 | sc-376408 AF680 | 200 µg/ml | $357.00 | |||
PLEKHA4 Antibody (H-1) Alexa Fluor® 790 | sc-376408 AF790 | 200 µg/ml | $357.00 | |||
PLEKHA4 (H-1) Neutralizing Peptide | sc-376408 P | 100 µg/0.5 ml | $68.00 |