Plectin Antibody (7A8): sc-58835

Plectin Antibody (7A8) is a mouse monoclonal IgG1 antibody that detects plectin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Plectin is a crucial cytoskeletal protein that plays a significant role in maintaining cellular integrity by crosslinking intermediate filaments to microtubules and microfilaments, as well as anchoring these filaments to the plasma and nuclear membranes. This structural support is vital for cellular stability and resilience, particularly in tissues that experience mechanical stress. Plectin′s interactions with other proteins, such as Lamin B and vimentin, are essential for its function, and these interactions are modulated by various protein kinases through phosphorylation. For instance, phosphorylation by protein kinase A (PKA) or protein kinase C (PKC) can reduce plectin′s binding affinity for Lamin B, while PKA-mediated phosphorylation enhances its interaction with vimentin. Additionally, plectin is a substrate for p34cdc2 kinase, highlighting its involvement in cell cycle regulation. The existence of several alternative splice isoforms of plectin further underscores its functional diversity. Importantly, mutations in human plectin have been linked to epidermolysis bullosa simplex with muscular dystrophy (EBS-MD), illustrating plectin′s critical role in human health. Anti-plectin antibody (7A8) is an invaluable tool for researchers studying the dynamics of cytoskeletal organization and the implications of plectin in various diseases.

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Plectin Antibody (7A8) References:

1. Determining the mechanical properties of plectin in mouse myoblasts and keratinocytes.  |  Bonakdar, N., et al. 2015. Exp Cell Res. 331: 331-7. PMID: 25447312
2. The rod domain is not essential for the function of plectin in maintaining tissue integrity.  |  Ketema, M., et al. 2015. Mol Biol Cell. 26: 2402-17. PMID: 25971800
3. Plectin reinforces vascular integrity by mediating crosstalk between the vimentin and the actin networks.  |  Osmanagic-Myers, S., et al. 2015. J Cell Sci. 128: 4138-50. PMID: 26519478
4. Plectin-1 Targeted Dual-modality Nanoparticles for Pancreatic Cancer Imaging.  |  Chen, X., et al. 2018. EBioMedicine. 30: 129-137. PMID: 29574092
5. Plectin Mutations in Progressive Familial Intrahepatic Cholestasis.  |  Wu, SH., et al. 2019. Hepatology. 70: 2221-2224. PMID: 31269534
6. Role of epigenetics in the pathogenesis and progression of osteoarthritis AK Sorial et al. Multi-tissue epigenetic analysis of the osteoarthritis susceptibility locus mapping to the plectin gene PLEC.  |  Malemud, CJ. 2020. Osteoarthritis Cartilage. 28: 1401-1402. PMID: 32891768
7. Integrin α6β4 requires plectin and vimentin for adhesion complex distribution and invasive growth.  |  Qi, L., et al. 2022. J Cell Sci. 135: PMID: 34897465
8. Plectin Deficiency in Fibroblasts Deranges Intermediate Filament and Organelle Morphology, Migration, and Adhesion.  |  Zrelski, MM., et al. 2024. J Invest Dermatol. 144: 547-562.e9. PMID: 37716646
9. DOTA-Based Plectin-1 Targeted Contrast Agent Enables Detection of Pancreatic Cancer in Human Tissue.  |  Gazzi, T., et al. 2024. Angew Chem Int Ed Engl. 63: e202318485. PMID: 38608197
10. Cancer cell extravasation requires iplectin-mediated delivery of MT1-MMP at invadopodia.  |  Grafinger, OR., et al. 2024. Br J Cancer. 131: 931-943. PMID: 38969866

The preferred Plectin antibody is Plectin Antibody (10F6): sc-33649.