Pleckstrin Antibody (25): sc-136042

Pleckstrin Antibody (25) is a mouse monoclonal IgG1 kappa light chain antibody that detects Pleckstrin protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-Pleckstrin antibody (25) is available as the non-conjugated format. Pleckstrin plays a crucial role in cellular signaling, particularly in platelets and neutrophils, where Pleckstrin acts as a major substrate for protein kinase C (PKC) activation. Upon PKC activation, Pleckstrin undergoes rapid phosphorylation, which is essential for mediating inflammatory responses. Pleckstrin features a unique structure that includes two pleckstrin homology (PH) domains at N and C termini, which facilitate interactions with both proteins and lipids. This structural characteristic allows Pleckstrin to translocate to cellular membranes and interact with the cytoskeleton, thereby enhancing its role in targeting proteins or lipids to sites of action during immune responses. Pleckstrin is highly expressed in human neutrophils and is phosphorylated in response to inflammatory stimuli, likely by nonconventional PKC isoforms such as delta or zeta. The conformational changes induced by phosphorylation enable Pleckstrin to effectively contribute to the microbicidal response, underscoring its importance in immune function.

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Pleckstrin Antibody (25) References:

1. cDNA cloning and mapping of mouse pleckstrin (Plek), a gene upregulated in transformation-resistant cells.  |  Cmarik, JL., et al. 2000. Genomics. 66: 204-12. PMID: 10860665
2. Molecular analysis of pleckstrin: the major protein kinase C substrate of platelets.  |  Tyers, M., et al. 1989. J Cell Biochem. 40: 133-45. PMID: 2768345
3. Molecular cloning and expression of the major protein kinase C substrate of platelets.  |  Tyers, M., et al. 1988. Nature. 333: 470-3. PMID: 2897630
4. Protein kinase C regulates pleckstrin by phosphorylation of sites adjacent to the N-terminal pleckstrin homology domain.  |  Abrams, CS., et al. 1995. J Biol Chem. 270: 23317-21. PMID: 7559487
5. Solution structure of a pleckstrin-homology domain.  |  Yoon, HS., et al. 1994. Nature. 369: 672-5. PMID: 8208296
6. Phosphorylation of human pleckstrin on Ser-113 and Ser-117 by protein kinase C.  |  Craig, KL. and Harley, CB. 1996. Biochem J. 314 (Pt 3): 937-42. PMID: 8615792
7. Phosphorylation and subcellular redistribution of pleckstrin in human neutrophils.  |  Brumell, JH., et al. 1997. J Immunol. 158: 4862-71. PMID: 9144502

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pleckstrin Antibody (25) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Pleckstrin Antibody (25) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pleckstrin Antibody (25) Specifications

Alternate Product Name: Pleckstrin

Product Clone Name: 25

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-16

Epitope Target: Pleckstrin

Epitope Species: human

Product Reactivity/Detection: Pleckstrin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Pleckstrin Antibody (25): N/A