Reduced-serum medium suitable for transfections with DNA Plasmids
Recommended for use with Plasmid Transfection Reagent (sc-108061) and suspension of DNA for transfection including: shRNA Plasmids, CRISPR/Cas9 KO Plasmids, HDR Plasmids or Cre Vector
Modification of Eagle's Minimal Essential Medium, buffered with HEPES and sodium bicarbonate, and supplemented with hypoxanthine, thymidine, sodium pyruvate, L-glutamine, trace elements and growth factors; protein level is minimal with insulin and transferrin being the only protein supplements; phenol red included at a reduced concentration as a pH indicator