phosvitin Antibody (D-5): sc-46681
- phosvitin Antibody (D-5) is a mouse monoclonal IgG2a κ phosvitin antibody provided at 200 µg/ml
- raised against full length phosvitin protein purified from egg yolk
- phosvitin Antibody (D-5) is recommended for detection of phosvitin of avian origin by WB, IP, IF and ELISA
- Anti-phosvitin Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for phosvitin Antibody (D-5) for WB applications. These reagents are now offered in bundles with phosvitin Antibody (D-5) (see ordering information below).
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Phosvitin Antibody (D-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects phosvitin protein of avian origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-phosvitin antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Phosvitin, also known as phosphovitin, is one of the most highly phosphorylated proteins found in nature, primarily located in the egg yolk of vertebrates, where phosvitin serves as a crucial component for embryonic development. This phosphoglycoprotein is synthesized in the liver as a fragment of vitellogenin, a precursor protein found in the blood of egg-laying animals. Phosvitin consists of 216 amino acids, with over 123 serine residues, which account for more than 50% of total amino acid composition; the extensive phosphorylation of these serine residues indicates phosvitin′s potential utility as a standard for phosphoserine studies. Notably, phosvitin′s structure lacks α-helix and β-sheet conformations under neutral pH conditions but transitions to a β-sheet structure in acidic environments. This structural adaptability allows phosvitin to function effectively as a metal transporter and iron reservoir, playing a vital role in supplying essential nutrients to developing embryos.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
phosvitin Antibody (D-5) References:
- Post-translational phosphorylation of phosvitin. | Schirm, J., et al. 1973. FEBS Lett. 30: 167-169. PMID: 11947085
- Egg yolk phosvitin: preparation of metal-free purified protein by fast protein liquid chromatography using aqueous solvents. | Castellani, O., et al. 2003. J Chromatogr B Analyt Technol Biomed Life Sci. 791: 273-84. PMID: 12798187
- Egg yolk phosvitin inhibits hydroxyl radical formation from the fenton reaction. | Ishikawa, S., et al. 2004. Biosci Biotechnol Biochem. 68: 1324-31. PMID: 15215598
- A Raman spectroscopic study of hen egg yolk phosvitin: structures in solution and in the solid state. | Prescott, B., et al. 1986. Biochemistry. 25: 2792-8. PMID: 3718921
- Phosvitin, a phosphoglycoprotein: composition and partial structure of carbohydrate moiety. | Shainkin, R. and Perlmann, GE. 1971. Arch Biochem Biophys. 145: 693-700. PMID: 4331032
- Phosvitin, a phosphoglycoprotein. I. Isolation and characterization of a glycopeptide from phosvitin. | Shainkin, R. and Perlmann, GE. 1971. J Biol Chem. 246: 2278-84. PMID: 5103536
- Amino acid sequence of phosvitin derived from the nucleotide sequence of part of the chicken vitellogenin gene. | Byrne, BM., et al. 1984. Biochemistry. 23: 4275-9. PMID: 6091745
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
phosvitin Antibody (D-5) | sc-46681 | 200 µg/ml | $316.00 | |||
phosvitin Antibody (D-5): m-IgG Fc BP-HRP Bundle | sc-525549 | 200 µg Ab; 10 µg BP | $354.00 | |||
phosvitin Antibody (D-5): m-IgG2a BP-HRP Bundle | sc-546310 | 200 µg Ab; 10 µg BP | $354.00 | |||
phosvitin Antibody (D-5) AC | sc-46681 AC | 500 µg/ml, 25% agarose | $416.00 | |||
phosvitin Antibody (D-5) HRP | sc-46681 HRP | 200 µg/ml | $316.00 | |||
phosvitin Antibody (D-5) FITC | sc-46681 FITC | 200 µg/ml | $330.00 | |||
phosvitin Antibody (D-5) PE | sc-46681 PE | 200 µg/ml | $343.00 | |||
phosvitin Antibody (D-5) Alexa Fluor® 488 | sc-46681 AF488 | 200 µg/ml | $357.00 | |||
phosvitin Antibody (D-5) Alexa Fluor® 546 | sc-46681 AF546 | 200 µg/ml | $357.00 | |||
phosvitin Antibody (D-5) Alexa Fluor® 594 | sc-46681 AF594 | 200 µg/ml | $357.00 | |||
phosvitin Antibody (D-5) Alexa Fluor® 647 | sc-46681 AF647 | 200 µg/ml | $357.00 | |||
phosvitin Antibody (D-5) Alexa Fluor® 680 | sc-46681 AF680 | 200 µg/ml | $357.00 | |||
phosvitin Antibody (D-5) Alexa Fluor® 790 | sc-46681 AF790 | 200 µg/ml | $357.00 |