PKR CRISPR Plasmids (h) human PKR-specific CRISPR/Cas9 KO Plasmid, HDR Plasmid, Double Nickase Plasmids, CRISPR Activation Plasmids and Lentiviral Activation Particles

PKR CRISPR Plasmids (h)

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Datasheets

    • Target species: human; for corresponding mouse product, see PKR CRISPR Plasmids (m)
    • CRISPR/Cas9 KO Plasmids consists of PKR-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
    • For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
    • Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
    • Gene knockdown or activation can be assayed using PKR Antibody (B-10): sc-6282
    • All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells

Gene Info

SpeciesGene NameGene IDChromosome LocationIsoform (mRNA) Accession #Protein Accession #OMIM™ Number
HumanEIF2AK256102p22.2XM_011532987, XM_017004503, NM_001135651, NM_001135652, NM_002759P19525176871

Query for Cat. No. sc-400177, sgRNA for PKR; Can you provide the location of sgRNA in the exon of PKR? As per information, three sgRNAs sequence have been combined to form one specific sgRNA for generating PKR KO.

Asked by: namsa
Thank you for your question. Please contact our Technical Service Department and we can provide you with the targeted exon information for this specific product. sc-400177: PKR CRISPR/Cas9 KO Plasmid (h) is provided as a pool of three different gRNA sequences. The three different gRNA are combined (pooled) together into a single vial of product to be used for transfection and KO. However, they do not form one specific gRNA. The purpose of providing three different gRNA is to increase the efficiency of generating a PKR KO with a single transfection.
Answered by: Technical Support
Date published: 2019-02-25
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