PKR Antibody (H-12): sc-514626

PKR Antibody (H-12) is a mouse monoclonal IgG1 antibody that detects PKR in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PKR, or interferon-inducible RNA-dependent protein serine/threonine kinase, plays a crucial role in the cellular response to viral infections by phosphorylating the α subunit of the protein synthesis initiation factor eIF-2. This phosphorylation event on serine-51 leads to the inhibition of translation, effectively halting viral replication and allowing the host cell to mount an immune response. PKR is primarily located in the cytoplasm, where PKR can rapidly respond to the presence of double-stranded RNA, a common indicator of viral infection. The structural organization of PKR is significant, as PKR catalytic domains are situated in the carboxy terminal half, while the amino terminal region contains RNA-binding domains, enabling PKR to sense viral RNA and activate kinase activity. Additionally, PKR is subject to complex regulatory mechanisms, including transcriptional regulation in response to interferon, an autoregulatory mechanism that modulates expression at the translational level, and post-translational modifications such as RNA-mediated autophosphorylation, which further fine-tune activity. Anti-PKR antibody (H-12) is an essential tool for researchers studying the intricate pathways of immune response and viral pathogenesis.

The preferred PKR antibody is PKR Antibody (B-10): sc-6282.