PKM Antibody (YY-3): sc-100538

PKM Antibody (YY-3) is a mouse monoclonal IgM antibody that detects PKM in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-PKM antibody (YY-3) is available in a non-conjugated form, providing researchers with a versatile tool for studying pyruvate kinase M1/2. PKM (YY-3) antibody enables research into PKM′s crucial role in cellular metabolism, specifically in glycolysis, where PKM catalyzes the conversion of phosphoenolpyruvate to pyruvate, generating adenosine triphosphate (ATP) in the process. This function is vital for energy production in cells, particularly in tissues with high metabolic demands such as muscle and brain. The PKM gene encodes two isoforms, M1 and M2, through alternative splicing, and both isoforms can form tetramers that are activated by fructose 1,6-bisphosphate. Notably, the M2-type isoform has been shown to interact with Oct-4, a key transcription factor in stem cell biology, enhancing its transcriptional activity and thereby influencing cellular differentiation and proliferation. This interaction underscores PKM′s importance not only in metabolism but also in developmental processes, making PKM (YY-3) monoclonal antibody an essential reagent for researchers investigating both metabolic pathways and stem cell dynamics.

The preferred PKM antibody is PKM Antibody (C-11): sc-365684.