PKM Antibody (YY-3): sc-100538
- PKM Antibody (YY-3) is a mouse monoclonal IgM, cited in 2 publications, provided at 200 µl ascites
- raised against recombinant PKM of human origin
- recommended for detection of PKM of mouse, rat and human origin by WB, IP and ELISA
- See PKM (C-11): sc-365684 for PKM antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PKM Antibody (YY-3). This work is in progress.
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PKM Antibody (YY-3) is a mouse monoclonal IgM antibody that detects PKM in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-PKM antibody (YY-3) is available in a non-conjugated form, providing researchers with a versatile tool for studying pyruvate kinase M1/2. PKM (YY-3) antibody enables research into PKM′s crucial role in cellular metabolism, specifically in glycolysis, where PKM catalyzes the conversion of phosphoenolpyruvate to pyruvate, generating adenosine triphosphate (ATP) in the process. This function is vital for energy production in cells, particularly in tissues with high metabolic demands such as muscle and brain. The PKM gene encodes two isoforms, M1 and M2, through alternative splicing, and both isoforms can form tetramers that are activated by fructose 1,6-bisphosphate. Notably, the M2-type isoform has been shown to interact with Oct-4, a key transcription factor in stem cell biology, enhancing its transcriptional activity and thereby influencing cellular differentiation and proliferation. This interaction underscores PKM′s importance not only in metabolism but also in developmental processes, making PKM (YY-3) monoclonal antibody an essential reagent for researchers investigating both metabolic pathways and stem cell dynamics.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PKM Antibody (YY-3) References:
- Regulation of glycolysis and gluconeogenesis by acetylation of PKM and PEPCK. | Xiong, Y., et al. 2011. Cold Spring Harb Symp Quant Biol. 76: 285-9. PMID: 22096030
- ASO-Based PKM Splice-Switching Therapy Inhibits Hepatocellular Carcinoma Growth. | Ma, WK., et al. 2022. Cancer Res. 82: 900-915. PMID: 34921016
- Linc-UROD stabilizes ENO1 and PKM to strengthen glycolysis, proliferation and migration of pancreatic cancer cells. | He, Y., et al. 2023. Transl Oncol. 27: 101583. PMID: 36413861
- The emerging role of PKM in keratinocyte homeostasis and pathophysiology. | Demeter, JB., et al. 2023. FEBS J. 290: 2311-2319. PMID: 36541050
- CD8+ T cell metabolic flexibility elicited by CD28-ARS2 axis-driven alternative splicing of PKM supports antitumor immunity. | Holling, GA., et al. 2024. Cell Mol Immunol. 21: 260-274. PMID: 38233562
- LINC01852 inhibits the tumorigenesis and chemoresistance in colorectal cancer by suppressing SRSF5-mediated alternative splicing of PKM. | Bian, Z., et al. 2024. Mol Cancer. 23: 23. PMID: 38263157
- The Regulatory Network of hnRNPs Underlying Regulating PKM Alternative Splicing in Tumor Progression. | Li, Y., et al. 2024. Biomolecules. 14: PMID: 38785973
- N6-methyladenosine-modified SRPK1 promotes aerobic glycolysis of lung adenocarcinoma via PKM splicing. | Wang, A., et al. 2024. Cell Mol Biol Lett. 29: 106. PMID: 39095708
- HNRNPC modulates PKM alternative splicing via m6A methylation, upregulating PKM2 expression to promote aerobic glycolysis in papillary thyroid carcinoma and drive malignant progression. | Rong, S., et al. 2024. J Transl Med. 22: 914. PMID: 39380010
- SARS-CoV-2 nucleocapsid protein interaction with YBX1 displays oncolytic properties through PKM mRNA destabilization. | Chen, X., et al. 2024. Mol Cancer. 23: 248. PMID: 39506849
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKM Antibody (YY-3) | sc-100538 | 200 µl ascites | $339.00 |