Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PKDCC Double Nickase Plasmid (m): sc-430656-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PKDCC Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PKDCC Double Nickase Plasmid (m) and PKDCC Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Pkdcc. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PKDCC Antibody (G-10): sc-514504
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKDCC Double Nickase Plasmid (m) | sc-430656-NIC | 20 µg | $410.00 |
Pkdcc encodes PKDCC, a secreted or Golgi-associated atypical serine/threonine protein kinase implicated in regulating extracellular signaling environments during development. PKDCC has been linked to control of cell–cell communication and morphogen-related pathways that influence proliferation and differentiation, with reported connections to Hedgehog signaling and skeletal patterning programs. In mouse systems, perturbation of Pkdcc function is used to study mechanisms underlying craniofacial and bone development, as well as broader kinase-dependent regulation of tissue morphogenesis. These features make PKDCC a useful node for interrogating how secreted kinase activity shapes signaling gradients and developmental phenotypes.
PKDCC Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Pkdcc locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Pkdcc. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Pkdcc function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Pkdcc-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.