PIWIL4 Antibody (10G9B11): sc-517215
- PIWIL4 Antibody (10G9B11) is a mouse monoclonal IgG1 PIWIL4 antibody provided at 50 µg/0.5 ml
- raised against a recombinant protein corresponding to amino acids 304-434 of PIWIL4 of human origin
- recommended for detection of PIWIL4 of human origin by WB, IP, IF, IHC(P), FCM and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PIWIL4 Antibody (10G9B11). This work is in progress.
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PIWIL4 Antibody (10G9B11) is a mouse monoclonal IgG1 antibody that detects PIWIL4 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). PIWIL4 (10G9B11) antibody is available as the non-conjugated form. PIWIL4, also known as HIWI2, is an 852 amino acid member of the argonaute family, characterized by the presence of one PAZ domain and one PIWI domain, which are crucial for RNA silencing and gene regulation. PIWIL4 plays a vital role in maintaining germline stem cells, particularly in adult testis where PIWIL4 is predominantly expressed. PIWIL4 is essential for regulating spermatogenesis and producing primordial germ cells, contributing significantly to meiotic differentiation of spermatocytes and self-renewal of spermatogonial stem cells. Studies have shown that PIWIL4-null males exhibit infertility due to defects in meiotic progression during early prophase of meiosis I, alongside progressive loss of germ cells with age. These mutant males demonstrate elevated expression of LINE-1 and intracisternal A particle (IAP) element transcripts in germ cell lineages, highlighting PIWIL4′s importance in maintaining genomic stability and proper germ cell function. PIWIL4 gene is located on human chromosome 11, underscoring PIWIL4′s significance in human reproductive biology.
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PIWIL4 Antibody (10G9B11) References:
- Identification of eight members of the Argonaute family in the human genome. | Sasaki, T., et al. 2003. Genomics. 82: 323-30. PMID: 12906857
- Mili, a mammalian member of piwi family gene, is essential for spermatogenesis. | Kuramochi-Miyagawa, S., et al. 2004. Development. 131: 839-49. PMID: 14736746
- Stem cell protein Piwil2 modulates expression of murine spermatogonial stem cell expressed genes. | Lee, JH., et al. 2006. Mol Reprod Dev. 73: 173-9. PMID: 16261612
- Stem-cell protein Piwil2 is widely expressed in tumors and inhibits apoptosis through activation of Stat3/Bcl-XL pathway. | Lee, JH., et al. 2006. Hum Mol Genet. 15: 201-11. PMID: 16377660
- Derivation of male germ cells from bone marrow stem cells. | Nayernia, K., et al. 2006. Lab Invest. 86: 654-63. PMID: 16652109
- A novel class of small RNAs bind to MILI protein in mouse testes. | Aravin, A., et al. 2006. Nature. 442: 203-7. PMID: 16751777
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PIWIL4 Antibody (10G9B11) | sc-517215 | 50 µg/0.5 ml | $316.00 |