Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Pitx2 Double Nickase Plasmid (h): sc-401426-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pitx2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Pitx2 Double Nickase Plasmid (h) and Pitx2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PITX2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Pitx2 Antibody (H-1): sc-390457
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pitx2 Double Nickase Plasmid (h) | sc-401426-NIC | 20 µg | $410.00 | |||
Pitx2 Double Nickase Plasmid (h2) | sc-401426-NIC-2 | 20 µg | $410.00 |
PITX2 encodes Pitx2, a homeobox transcription factor that regulates cell fate decisions and tissue patterning during embryogenesis and adult homeostasis. Pitx2 functions downstream of Wnt/β-catenin and integrates with TGF-β and retinoic acid–responsive transcriptional programs to control proliferation, differentiation, and morphogenetic processes. In human biology, altered PITX2 expression or regulatory variation has been linked to developmental disorders involving ocular and craniofacial structures and to dysregulated transcriptional networks in cancer and cardiac electrophysiology contexts. These pathway connections make PITX2 a useful node for studying gene regulatory circuits, lineage specification, and context-dependent transcriptional control.
Pitx2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PITX2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PITX2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PITX2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PITX2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.