Pitx2 Antibody (H-1): sc-390457

Pitx2 Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Pitx2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Pitx2 antibody (H-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pitx2 plays a crucial role as a transcription factor in regulating various developmental processes, including cardiac positioning and the morphogenesis of the pituitary gland and teeth. Notably, Pitx2 is essential for establishing left-right asymmetry in the body, as Pitx2 is asymmetrically expressed in the left lateral-plate mesoderm during embryonic development. This asymmetric expression is vital for proper lung symmetry, and mutations in Pitx2 can lead to significant developmental disorders, such as Rieger syndrome, which is characterized by a range of congenital anomalies. Pitx2′s importance extends to interactions with other proteins involved in developmental pathways, highlighting Pitx2′s role in the intricate network of gene regulation that governs organ development and symmetry. The genes encoding Pitx1 and Pitx2 are located on human chromosomes 5q31 and 4q25-q26, respectively, underscoring their evolutionary conservation and functional significance across species.

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Pitx2 Antibody (H-1) References:

1. Role of the Bicoid-related homeodomain factor Pitx1 in specifying hindlimb morphogenesis and pituitary development.  |  Szeto, DP., et al. 1999. Genes Dev. 13: 484-94. PMID: 10049363
2. A model for the development of the hypothalamic-pituitary axis: transcribing the hypophysis.  |  Kioussi, C., et al. 1999. Mech Dev. 81: 23-35. PMID: 10330482
3. Limb development: Farewell to arms.  |  Graham, A. and McGonnell, I. 1999. Curr Biol. 9: R368-70. PMID: 10339420
4. Pitx2 regulates lung asymmetry, cardiac positioning and pituitary and tooth morphogenesis.  |  Lin, CR., et al. 1999. Nature. 401: 279-82. PMID: 10499586
5. Xpitx-1: a homeobox gene expressed during pituitary and cement gland formation of Xenopus embryos.  |  Hollemann, T. and Pieler, T. 1999. Mech Dev. 88: 249-52. PMID: 10534625
6. PITX2-dependent gene regulation in atrial fibrillation and rhythm control.  |  Syeda, F., et al. 2017. J Physiol. 595: 4019-4026. PMID: 28217939
7. Understanding PITX2-Dependent Atrial Fibrillation Mechanisms through Computational Models.  |  Bai, J., et al. 2021. Int J Mol Sci. 22: PMID: 34299303
8. Pitx2 patterns an accelerator-brake mechanical feedback through latent TGFβ to rotate the gut.  |  Sanketi, BD., et al. 2022. Science. 377: eabl3921. PMID: 36137018
9. PITX2 Knockout Induces Key Findings of Electrical Remodeling as Seen in Persistent Atrial Fibrillation.  |  Schulz, C., et al. 2023. Circ Arrhythm Electrophysiol. 16: e011602. PMID: 36763906
10. PITX2 induction leads to impaired cardiomyocyte function in arrhythmogenic cardiomyopathy.  |  van Kampen, SJ., et al. 2023. Stem Cell Reports. 18: 749-764. PMID: 36868229
11. Pituitary homeobox 2, a novel member of the bicoid-related family of homeobox genes, is a potential regulator of anterior structure formation.  |  Gage, PJ. and Camper, SA. 1997. Hum Mol Genet. 6: 457-64. PMID: 9147650
12. Human and murine PTX1/Ptx1 gene maps to the region for Treacher Collins syndrome.  |  Crawford, MJ., et al. 1997. Mamm Genome. 8: 841-5. PMID: 9337397

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pitx2 Antibody (H-1) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Pitx2 Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pitx2 Antibody (H-1) Specifications

Alternate Product Name: Pitx2

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 246-262

Epitope Target: Pitx2B

Epitope Species: human

Product Reactivity/Detection: Pitx2A, Pitx2B and Pitx2C

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Pitx2 Antibody (H-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent