Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PiT1 CRISPR/Cas9 KO Plasmid (h): sc-403121
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PiT1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PiT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PiT1 CRISPR/Cas9 KO Plasmid (h) | sc-403121 | 20 µg | $397.00 |
Overview
SLC20A1 encodes PiT1, a ubiquitously expressed type III sodium-dependent phosphate symporter that regulates inorganic phosphate uptake to support nucleotide synthesis, membrane phospholipid production, and cellular bioenergetics. PiT1 influences phosphate homeostasis–linked signaling networks, including ERK/MAPK and cell-cycle control, and is implicated in osteogenic differentiation and mineralization processes through phosphate-sensitive transcriptional programs. Altered SLC20A1 activity has been associated with dysregulated proliferation and stress responses and is frequently studied in the context of vascular calcification, metabolic remodeling, and tumor biology where phosphate availability can shape growth and survival phenotypes.
PiT1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC20A1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC20A1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC20A1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PiT1 protein expression.
This CRISPR knockout system enables efficient generation of SLC20A1-deficient cell models for investigation of PiT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC20A1 exon(s) critical for PiT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC20A1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PiT1 CRISPR/Cas9 KO Plasmid (h) and PiT1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC20A1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PiT1 HDR Plasmid (h) and PiT1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC20A1 homology arms to support homology-directed repair at defined SLC20A1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.