PIST Antibody (A-7): sc-393026
- PIST Antibody (A-7) is a mouse monoclonal IgG2b κ PIST antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 158-291 mapping within an internal region of PIST of human origin
- PIST Antibody (A-7) is recommended for detection of PIST of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
- Anti-PIST Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for PIST Antibody (A-7) for WB applications. This reagent is now offered in a bundle with PIST Antibody (A-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
QUICK LINKS
SEE ALSO...
PIST Antibody (A-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects PIST protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PIST (A-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PIST protein, also known as PDZ protein interacting specifically with TC10, GOPC, CAL, or FIG, is a 462 amino acid protein primarily localized in the cytoplasm and Golgi apparatus membrane, as well as at cell junctions. Such localization facilitates PIST′s role in intracellular protein trafficking and degradation, ensuring proteins are correctly sorted and delivered to their destinations within the cell. PIST contains one PDZ (DHR) domain and functions as a homooligomer, interacting with various proteins to modulate cellular processes. PIST is implicated in ionic current regulation through membrane channel modification and may play a significant role in autophagy. Chromosomal aberrations in PIST-encoding genes have been associated with glioblastoma multiforme, an aggressive brain tumor, suggesting a potential link between mutated PIST and carcinogenesis. Three PIST isoforms exist due to alternative splicing events, contributing to diverse functional roles in cellular physiology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PIST Antibody (A-7) References:
- Identification of a PDZ domain containing Golgi protein, GOPC, as an interaction partner of frizzled. | Yao, R., et al. 2001. Biochem Biophys Res Commun. 286: 771-8. PMID: 11520064
- Modulation of mature cystic fibrosis transmembrane regulator protein by the PDZ domain protein CAL. | Cheng, J., et al. 2004. J Biol Chem. 279: 1892-8. PMID: 14570915
- Interaction with cystic fibrosis transmembrane conductance regulator-associated ligand (CAL) inhibits beta1-adrenergic receptor surface expression. | He, J., et al. 2004. J Biol Chem. 279: 50190-6. PMID: 15358775
- Isoform-specific interaction of golgin-160 with the Golgi-associated protein PIST. | Hicks, SW. and Machamer, CE. 2005. J Biol Chem. 280: 28944-51. PMID: 15951434
- Interactions with PDZ domain proteins PIST/GOPC and PDZK1 regulate intracellular sorting of the somatostatin receptor subtype 5. | Wente, W., et al. 2005. J Biol Chem. 280: 32419-25. PMID: 16012170
- Identification of a PDZ protein, PIST, as a binding partner for Rho effector Rhotekin: biochemical and cell-biological characterization of Rhotekin-PIST interaction. | Ito, H., et al. 2006. Biochem J. 397: 389-98. PMID: 16646955
- Solution structure of GOPC PDZ domain and its interaction with the C-terminal motif of neuroligin. | Li, X., et al. 2006. Protein Sci. 15: 2149-58. PMID: 16882988
- The relative binding affinities of PDZ partners for CFTR: a biochemical basis for efficient endocytic recycling. | Cushing, PR., et al. 2008. Biochemistry. 47: 10084-98. PMID: 18754678
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PIST Antibody (A-7) | sc-393026 | 200 µg/ml | $316.00 | |||
PIST Antibody (A-7): m-IgGκ BP-HRP Bundle | sc-523778 | 200 µg Ab, 40 µg BP | $354.00 | |||
PIST Antibody (A-7) AC | sc-393026 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PIST Antibody (A-7) HRP | sc-393026 HRP | 200 µg/ml | $316.00 | |||
PIST Antibody (A-7) FITC | sc-393026 FITC | 200 µg/ml | $330.00 | |||
PIST Antibody (A-7) PE | sc-393026 PE | 200 µg/ml | $343.00 | |||
PIST Antibody (A-7) Alexa Fluor® 488 | sc-393026 AF488 | 200 µg/ml | $357.00 | |||
PIST Antibody (A-7) Alexa Fluor® 546 | sc-393026 AF546 | 200 µg/ml | $357.00 | |||
PIST Antibody (A-7) Alexa Fluor® 594 | sc-393026 AF594 | 200 µg/ml | $357.00 | |||
PIST Antibody (A-7) Alexa Fluor® 647 | sc-393026 AF647 | 200 µg/ml | $357.00 | |||
PIST Antibody (A-7) Alexa Fluor® 680 | sc-393026 AF680 | 200 µg/ml | $357.00 | |||
PIST Antibody (A-7) Alexa Fluor® 790 | sc-393026 AF790 | 200 µg/ml | $357.00 |