PIS1 Antibody (C-2): sc-514255
- PIS1 Antibody (C-2) is a mouse monoclonal IgG2a κ PIS1 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 55-78 within an internal region of PIS1 of human origin
- PIS1 Antibody (C-2) is recommended for detection of PIS1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PIS1 Antibody (C-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PIS1 Antibody (C-2) for WB applications. These reagents are now offered in bundles with PIS1 Antibody (C-2) (see ordering information below).
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PIS1 Antibody (C-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects PIS1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PIS1 (C-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PIS1, also known as CDIPT (CDP-diacylglycerol--inositol 3-phosphatidyltransferase) or phosphatidylinositol synthase, is a 213 amino acid multi-pass membrane protein that plays a crucial role in the biosynthesis of phosphatidylinositol (PtdIns), a key phospholipid involved in cellular signaling and membrane dynamics. PIS1 is primarily localized to the Golgi apparatus and the cytoplasmic side of the endoplasmic reticulum, with highest expression levels found in adult liver and skeletal muscle, highlighting importance in metabolic processes. PIS1 exists as two alternatively spliced isoforms and is highly conserved across yeast and mammals, indicating essential function in cellular physiology. PIS1 is encoded by a gene located on human chromosome 16p11.2, and PIS1 activity is CMP-dependent, facilitating the PtdIns-inositol exchange reaction while also potentially regulating excessive cellular PtdIns levels.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PIS1 Antibody (C-2) References:
- Expression of the yeast PIS1 gene requires multiple regulatory elements including a Rox1p binding site. | Gardocki, ME. and Lopes, JM. 2003. J Biol Chem. 278: 38646-52. PMID: 12890676
- Genomic analysis of PIS1 gene expression. | Gardocki, ME., et al. 2005. Eukaryot Cell. 4: 604-14. PMID: 15755922
- Regulation of the PIS1-encoded phosphatidylinositol synthase in Saccharomyces cerevisiae by zinc. | Han, SH., et al. 2005. J Biol Chem. 280: 29017-24. PMID: 15980062
- Novel molecular targets of smokeless tobacco (khaini) in cell culture from oral hyperplasia. | Rohatgi, N., et al. 2006. Toxicology. 224: 1-13. PMID: 16730401
- Clinical significance of phosphatidyl inositol synthase overexpression in oral cancer. | Kaur, J., et al. 2010. BMC Cancer. 10: 168. PMID: 20426864
- Genome-wide approaches to schizophrenia. | Duan, J., et al. 2010. Brain Res Bull. 83: 93-102. PMID: 20433910
- Effect of unsaturated fatty acids and Ca2+ on phosphatidylinositol synthesis and breakdown. | Takenawa, T. and Nagai, Y. 1982. J Biochem. 91: 793-9. PMID: 6281246
- Carbon source regulation of PIS1 gene expression in Saccharomyces cerevisiae involves the MCM1 gene and the two-component regulatory gene, SLN1. | Anderson, MS. and Lopes, JM. 1996. J Biol Chem. 271: 26596-601. PMID: 8900132
- PIS1, a negative regulator of the action of auxin transport inhibitors in Arabidopsis thaliana. | Fujita, H. and Syono, K. 1997. Plant J. 12: 583-95. PMID: 9351244
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PIS1 Antibody (C-2) | sc-514255 | 200 µg/ml | $322.00 | |||
PIS1 Antibody (C-2): m-IgG Fc BP-HRP Bundle | sc-526352 | 200 µg Ab; 10 µg BP | $361.00 | |||
PIS1 Antibody (C-2): m-IgG2a BP-HRP Bundle | sc-546601 | 200 µg Ab; 10 µg BP | $361.00 | |||
PIS1 Antibody (C-2) AC | sc-514255 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PIS1 Antibody (C-2) HRP | sc-514255 HRP | 200 µg/ml | $322.00 | |||
PIS1 Antibody (C-2) FITC | sc-514255 FITC | 200 µg/ml | $336.00 | |||
PIS1 Antibody (C-2) PE | sc-514255 PE | 200 µg/ml | $349.00 | |||
PIS1 Antibody (C-2) Alexa Fluor® 488 | sc-514255 AF488 | 200 µg/ml | $364.00 | |||
PIS1 Antibody (C-2) Alexa Fluor® 546 | sc-514255 AF546 | 200 µg/ml | $364.00 | |||
PIS1 Antibody (C-2) Alexa Fluor® 594 | sc-514255 AF594 | 200 µg/ml | $364.00 | |||
PIS1 Antibody (C-2) Alexa Fluor® 647 | sc-514255 AF647 | 200 µg/ml | $364.00 | |||
PIS1 Antibody (C-2) Alexa Fluor® 680 | sc-514255 AF680 | 200 µg/ml | $364.00 | |||
PIS1 Antibody (C-2) Alexa Fluor® 790 | sc-514255 AF790 | 200 µg/ml | $364.00 | |||
PIS1 (C-2) Neutralizing Peptide | sc-514255 P | 100 µg/0.5 ml | $69.00 |