PIPK I Antibody (D-12): sc-365238

PIPK I Antibody (D-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects PIPK I protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PIPK I (D-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The phosphatidylinositol-4-phosphate-5-kinase (PIPK) family plays a crucial role in synthesizing phosphatidylinositol-4,5-bisphosphate, a lipid vital for regulating numerous cellular processes such as cell proliferation, survival, membrane trafficking, and cytoskeletal organization. Within this family, PIPK I consists of three isoforms—PIPK I α, β, and γ—each phosphorylating distinct substrates and characterized by an activation loop that dictates enzymatic specificity and subcellular localization. PIPK I α, known as PIPK I β in mice, is predominantly expressed in brain tissue, while PIPK I β, referred to as PIPK I α in mice, is also identified as STM7. PIPK I γ has two variants generated through alternative splicing, expressed in the lung, brain, and kidneys, highlighting diverse functional roles in various tissues.

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PIPK I Antibody (D-12) References:

1. The activation loop of phosphatidylinositol phosphate kinases determines signaling specificity.  |  Kunz, J., et al. 2000. Mol Cell. 5: 1-11. PMID: 10678164
2. Autophosphorylation of type I phosphatidylinositol phosphate kinase regulates its lipid kinase activity.  |  Itoh, T., et al. 2000. J Biol Chem. 275: 19389-94. PMID: 10777481
3. Phosphatidylinositol 4-phosphate 5-kinase type I is regulated through phosphorylation response by extracellular stimuli.  |  Park, SJ., et al. 2001. J Biol Chem. 276: 4781-7. PMID: 11087761
4. Ubiquitylation of phosphatidylinositol 4-phosphate 5-kinase type I γ by HECTD1 regulates focal adhesion dynamics and cell migration.  |  Li, X., et al. 2013. J Cell Sci. 126: 2617-28. PMID: 23572508
5. Lovastatin-Induced Phosphatidylinositol-4-Phosphate 5-Kinase Diffusion from Microvilli Stimulates ROMK Channels.  |  Liu, BC., et al. 2015. J Am Soc Nephrol. 26: 1576-87. PMID: 25349201
6. p70S6K1 (S6K1)-mediated Phosphorylation Regulates Phosphatidylinositol 4-Phosphate 5-Kinase Type I γ Degradation and Cell Invasion.  |  Jafari, N., et al. 2016. J Biol Chem. 291: 25729-25741. PMID: 27780861
7. Cdk5-mediated phosphorylation regulates phosphatidylinositol 4-phosphate 5-kinase type I γ 90 activity and cell invasion.  |  Li, L., et al. 2019. FASEB J. 33: 631-642. PMID: 30040488
8. The cloning and sequence of the C isoform of PtdIns4P 5-kinase.  |  Divecha, N., et al. 1995. Biochem J. 309 (Pt 3): 715-9. PMID: 7639683
9. Type I phosphatidylinositol-4-phosphate 5-kinases are distinct members of this novel lipid kinase family.  |  Loijens, JC. and Anderson, RA. 1996. J Biol Chem. 271: 32937-43. PMID: 8955136
10. Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the third isoform and deletion/substitution analysis of members of this novel lipid kinase family.  |  Ishihara, H., et al. 1998. J Biol Chem. 273: 8741-8. PMID: 9535851
11. Type I phosphatidylinositol-4-phosphate 5-kinases synthesize the novel lipids phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 5-phosphate.  |  Tolias, KF., et al. 1998. J Biol Chem. 273: 18040-6. PMID: 9660759
12. Structure of type IIbeta phosphatidylinositol phosphate kinase: a protein kinase fold flattened for interfacial phosphorylation.  |  Rao, VD., et al. 1998. Cell. 94: 829-39. PMID: 9753329

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PIPK I Antibody (D-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PIPK I Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PIPK I Antibody (D-12) Specifications

Alternate Product Name: PIPK I

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: PIPK I γ

Epitope Species: human

Product Reactivity/Detection: PIPK I α, β and γ

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PIPK I Antibody (D-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin