PIPK I α Antibody (B-10): sc-376634

PIPK I α Antibody (B-10) is a mouse monoclonal IgG1 antibody that detects phosphatidylinositol-4-phosphate-5-kinase I α (PIPK I α) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PIPK I α plays a crucial role in synthesizing phosphatidylinositol-4,5-bisphosphate, a key lipid that regulates various cellular processes, including cell proliferation, survival, and cytoskeletal organization. PIPK I α phosphorylates phosphatidylinositol 4-phosphate at the 5-hydroxyl position, which is essential for generating signaling molecules that mediate cellular responses to external stimuli. PIPK I α is predominantly expressed in brain tissue, where PIPK I α contributes to neuronal signaling and synaptic function. Additionally, PIPK I α is part of a larger family of PIPK enzymes, which are categorized into type I, type II, and type III, each with distinct substrate specificities and regulatory mechanisms. The presence of an activation loop within these kinases is critical for enzymatic activity and subcellular localization, underscoring the intricate regulation of lipid signaling pathways in health and disease. Anti-PIPK I α antibody (B-10) is an invaluable tool for researchers investigating the roles of phosphoinositides in cellular dynamics and signaling pathways.

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PIPK I α Antibody (B-10) References:

1. A novel testis ubiquitin-binding protein gene arose by exon shuffling in hominoids.  |  Babushok, DV., et al. 2007. Genome Res. 17: 1129-38. PMID: 17623810
2. Resolution of structure of PIP5K1A reveals molecular mechanism for its regulation by dimerization and dishevelled.  |  Hu, J., et al. 2015. Nat Commun. 6: 8205. PMID: 26365782
3. Regulation of HGF-induced hepatocyte proliferation by the small GTPase Arf6 through the PIP2-producing enzyme PIP5K1A.  |  Tsai, MT., et al. 2017. Sci Rep. 7: 9438. PMID: 28842595
4. Interrogating the protein interactomes of RAS isoforms identifies PIP5K1A as a KRAS-specific vulnerability.  |  Adhikari, H. and Counter, CM. 2018. Nat Commun. 9: 3646. PMID: 30194290
5. Membrane Recognition and Binding by the Phosphatidylinositol Phosphate Kinase PIP5K1A: A Multiscale Simulation Study.  |  Amos, STA., et al. 2019. Structure. 27: 1336-1346.e2. PMID: 31204251
6. Decrease in Multidrug Resistance-associated Protein 2 Activities by Knockdown of Phosphatidylinositol 4-phosphate 5-kinase in Hepatocytes and Cancer Cells.  |  Kawase, A., et al. 2019. J Pharm Pharm Sci. 22: 576-584. PMID: 31804920
7. Phosphatidylinositol-4-phosphate 5-kinase type 1α attenuates Aβ production by promoting non-amyloidogenic processing of amyloid precursor protein.  |  Wu, PF., et al. 2020. FASEB J. 34: 12127-12146. PMID: 32686865
8. Exosomal circ_PIP5K1A regulates the progression of non-small cell lung cancer and cisplatin sensitivity by miR-101/ABCC1 axis.  |  Shao, N., et al. 2021. Mol Cell Biochem. 476: 2253-2267. PMID: 33570734
9. Circ_PIP5K1A regulates cisplatin resistance and malignant progression in non-small cell lung cancer cells and xenograft murine model via depending on miR-493-5p/ROCK1 axis.  |  Feng, N., et al. 2021. Respir Res. 22: 248. PMID: 34537072
10. Dietary interventions and molecular mechanisms for healthy musculoskeletal aging.  |  Murphy, A., et al. 2022. Biogerontology. 23: 681-698. PMID: 35727468

The preferred PIPK I antibody is PIPK I Antibody (D-12): sc-365238.